Institute of Photonics and Optoelectronics, and Department of Electrical Engineering, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
Institute of Micro/Nano Photonic Materials and Application, School of Physics and Electronics, Henan University, Kaifeng 475004, China.
Molecules. 2018 Nov 30;23(12):3157. doi: 10.3390/molecules23123157.
The methods of cell perforation and preheating are used for increasing cell uptake efficiencies of gold nanorings (NRIs), which have the localized surface plasmon resonance wavelength around 1064 nm, and photosensitizer, AlPcS, and hence enhancing the cell damage efficiency through the photothermal (PT) and photodynamic (PD) effects. The perforation and preheating effects are generated by illuminating a defocused 1064-nm femtosecond (fs) laser and a defocused 1064-nm continuous (cw) laser, respectively. Cell damage is produced by illuminating cell samples with a focused 1064-nm cw laser through the PT effect, a focused 1064-nm fs laser through both PT and PD effects, and a focused 660-nm cw laser through the PD effect. Under various conditions with and without cell wash before laser illumination, through either perforation or preheating process, cell uptake and hence cell damage efficiencies can be enhanced. Under our experimental conditions, perforation can be more effective at enhancing cell uptake and damage when compared with preheating.
细胞穿孔和预热方法用于提高金纳米环（NRIs）的细胞摄取效率，其局部表面等离激元共振波长约为 1064nm，并与光敏剂 AlPcS 结合，从而通过光热（PT）和光动力（PD）效应增强细胞损伤效率。穿孔和预热效果分别通过照射聚焦的 1064nm 飞秒（fs）激光和聚焦的 1064nm 连续（cw）激光产生。通过光热效应，用聚焦的 1064nm cw 激光照射细胞样品产生细胞损伤，通过聚焦的 1064nm fs 激光通过 PT 和 PD 效应产生细胞损伤，以及通过聚焦的 660nm cw 激光通过 PD 效应产生细胞损伤。在有和没有激光照射前细胞洗涤的各种条件下，通过穿孔或预热过程，可以增强细胞摄取，从而增强细胞损伤效率。在我们的实验条件下，与预热相比，穿孔在增强细胞摄取和损伤方面更有效。
