Chu Chih-Ken, Tu Yi-Chou, Hsiao Jen-Hung, Yu Jian-He, Yu Chih-Kang, Chen Shih-Yang, Tseng Po-Hao, Chen Shuai, Kiang Yean-Woei, Yang C C
Institute of Photonics and Optoelectronics, National Taiwan University, No. 1, section 4, Roosevelt Road, Taipei, 10617 Taiwan.
Nanotechnology. 2016 Mar 18;27(11):115102. doi: 10.1088/0957-4484/27/11/115102. Epub 2016 Feb 15.
We demonstrate effective inactivation of oral cancer cells SAS through a combination of photothermal therapy (PTT) and photodynamic therapy (PDT) effects based on localized surface plasmon resonance (LSPR) around 1064 nm in wavelength of a Au nanoring (NRI) under femtosecond (fs) laser illumination. The PTT effect is caused by the LSPR-enhanced absorption of the Au NRI. The PDT effect is generated by linking the Au NRI with the photosensitizer of sulfonated aluminum phthalocyanines (AlPcS) for producing singlet oxygen through the LSPR-enhanced two-photon absorption (TPA) excitation of AlPcS. The laser threshold intensity for cancer cell inactivation with the applied Au NRI linked with AlPcS is significantly lower when compared to that with the Au NRI not linked with AlPcS. The comparison of inactivation threshold intensity between the cases of fs and continuous laser illuminations at the same wavelength and with the same average power confirms the crucial factor of TPA under fs laser illumination for producing the PDT effect.
我们证明，在飞秒（fs）激光照射下，基于波长约1064nm的金纳米环（NRI）周围的局域表面等离子体共振（LSPR），通过光热疗法（PTT）和光动力疗法（PDT）的联合效应，可有效灭活口腔癌细胞SAS。PTT效应由金NRI的LSPR增强吸收引起。PDT效应是通过将金NRI与磺化铝酞菁（AlPcS）光敏剂相连产生的，通过LSPR增强的AlPcS双光子吸收（TPA）激发产生单线态氧。与未与AlPcS相连的金NRI相比，与AlPcS相连的应用金NRI使癌细胞失活的激光阈值强度显著更低。在相同波长和相同平均功率下，fs激光照射与连续激光照射情况下失活阈值强度的比较证实了fs激光照射下TPA对产生PDT效应的关键作用。
