College of Transportation Engineering , Dalian Maritime University , Dalian 116026 , P. R. China.
Liaoning Province Key Laboratory of Oral Disease, School of Stomatology , China Medical University , Shenyang 110002 , P. R. China.
ACS Appl Mater Interfaces. 2019 Jan 9;11(1):1595-1603. doi: 10.1021/acsami.8b16547. Epub 2018 Dec 17.
Gradient biomaterials have shown enormous potential in high-throughput screening of biomaterials and material-induced cell migration. To make the screening process more rapid and precise, improving the regularity of morphological structure and chemical modification on gradient biomaterials have attracted much attention. In this paper, we present a novel fabrication strategy to introduce ordered nanopattern arrays into gradient biomaterials, through combining surface-initiated atom transfer radical polymerization and inclined reactive-ion etching based on colloidal lithography. Graded protein/poly(ethylene glycol) (PEG) nanopattern arrays on a quartz substrate were fabricated and applied to affect the behaviors of cells. Owing to the continuously changed ratio of two different components, the corresponding cell adhesion density along the substrate showed obvious graded distribution after culturing for 24 h. Meanwhile, the cytoskeleton showed obvious polarization after culturing for 7 days, which is parallel with the direction of gradient. Additionally, oriented migration was generated when mouse MC3T3-E1 cells were cultured on the graded protein/PEG nanopattern arrays. On the basis of the ordered and well-defined nanopatterns, the correlation between the extracellular matrix and corresponding expressions generated by different stimuli can be investigated.
梯度生物材料在高通量筛选生物材料和材料诱导的细胞迁移方面显示出了巨大的潜力。为了使筛选过程更加快速和精确，提高梯度生物材料的形态结构和化学修饰的规则性引起了广泛关注。在本文中，我们提出了一种将有序纳米图案引入梯度生物材料的新制造策略，该策略通过结合表面引发原子转移自由基聚合和基于胶体光刻的倾斜反应离子刻蚀来实现。在石英衬底上制备了分级蛋白/聚（乙二醇）（PEG）纳米图案阵列，并将其用于影响细胞的行为。由于两种不同成分的比例不断变化，培养 24 小时后，基底上相应的细胞黏附密度呈现明显的梯度分布。同时，培养 7 天后细胞骨架呈现明显的极化，与梯度方向平行。此外，当鼠 MC3T3-E1 细胞在分级蛋白/PEG 纳米图案阵列上培养时，产生了定向迁移。基于有序且明确的纳米图案，可以研究不同刺激产生的细胞外基质与相应表达之间的相关性。
