Department of Biotechnology , Korea University , Seoul 02841 , Republic of Korea.
Institute of Life Science and Natural Resources , Korea University , Seoul 02841 , Republic of Korea.
J Agric Food Chem. 2018 Dec 26;66(51):13454-13463. doi: 10.1021/acs.jafc.8b05093. Epub 2018 Dec 14.
Taurine is a biologically and physiologically valuable food additive. However, commercial taurine production mainly relies on environmentally harmful chemical synthesis. Herein, for the first time in bacteria, we attempted to produce taurine in metabolically engineered Corynebacterium glutamicum. The taurine-producing strain was developed by introducing cs, cdo1, and csad genes. Interestingly, while the control strain could not produce taurine, the engineered strains successfully produced taurine via the newly introduced metabolic pathway. Furthermore, we investigated the effect of a deletion strain of the transcriptional repressor McbR gene on taurine production. As a result, sulfur accumulation and l-cysteine biosynthesis were reinforced by the McbR deletion strain, which further increased the taurine production by 2.3-fold. Taurine production of the final engineered strain Tau11 was higher than in other previously reported strains. This study demonstrated a potential approach for eco-friendly biosynthesis as an alternative to the chemical synthesis of a food additive.
牛磺酸是一种具有生物和生理价值的食品添加剂。然而，商业牛磺酸的生产主要依赖于对环境有害的化学合成。在此，我们首次在细菌中尝试在代谢工程化的谷氨酸棒杆菌中生产牛磺酸。通过引入 cs、cdo1 和 csad 基因，开发了生产牛磺酸的菌株。有趣的是，尽管对照菌株不能生产牛磺酸，但通过新引入的代谢途径，工程菌株成功地生产了牛磺酸。此外，我们研究了转录抑制剂 McbR 基因缺失菌株对牛磺酸生产的影响。结果表明，McbR 缺失菌株增强了硫积累和 l-半胱氨酸的生物合成，从而使牛磺酸的产量进一步提高了 2.3 倍。最终工程菌株 Tau11 的牛磺酸产量高于其他先前报道的菌株。这项研究为环保型生物合成提供了一种潜在的方法，可替代食品添加剂的化学合成。
