Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Environ Sci Pollut Res Int. 2020 Apr;27(12):13180-13193. doi: 10.1007/s11356-020-07895-1. Epub 2020 Feb 3.
Currently, hepatic injury due to environmental pollutants extremely threatens human health and elicits great concern. Hence, there is a high global interest to find natural novel formulation products with potent hepatoprotective activity to combat liver disease. Hence, we evaluated the protective or therapeutic effect of hesperidin (HSP) and taurine (TAU), individually and in combination, on carbon tetrachloride (CCl)-induced acute hepatic injury in rats. The pre- or posttreatment by HSP and/or TAU significantly depressed CCl-induced elevation of alanine aminotransferase, alkaline phosphatase, aspartate aminotransferase, gamma-glutamyl transferase, total bilirubin, direct bilirubin, indirect bilirubin, malondialdehyde, globulins (α1, α2, β, and γ), albumin/globulin ratio, triglycerides, total cholesterol, high-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, low-density lipoprotein cholesterol, nitric oxide, and myeloperoxidase levels. Also, the pre- or posttreatment by HSP and/or TAU significantly minimized CCl-induced reduction of superoxide dismutase, catalase, reduced glutathione, and albumin concentrations. Furthermore, the protective or therapeutic administration of HSP and/or TAU markedly restored the CCl-induced altered hepatic architecture, depleted glycogen, and DNA contents. Notably, alleviating CCl-induced hepatotoxicity was more prominent in the protective groups than the therapeutic groups. More importantly, most of biochemical and histopathological parameters of HSP+TAU did not significantly differ from those of separate TAU or HSP neither before nor after CCl exposure. Conclusively, HSP or TAU could be candidate protective agents against CCl hepatotoxic impacts but the combination of both bioactive offers only a limited synergistic effect. Graphical abstract.
目前，环境污染物引起的肝损伤极大地威胁着人类健康，引起了极大的关注。因此，全球都有很高的兴趣寻找具有潜在保肝活性的天然新型制剂产品来对抗肝脏疾病。因此，我们评估了橙皮苷（HSP）和牛磺酸（TAU）单独和联合应用对四氯化碳（CCl）诱导的大鼠急性肝损伤的保护或治疗作用。HSP 和/或 TAU 的预处理或后处理显著降低了 CCl 诱导的丙氨酸氨基转移酶、碱性磷酸酶、天冬氨酸氨基转移酶、γ-谷氨酰转移酶、总胆红素、直接胆红素、间接胆红素、丙二醛、球蛋白（α1、α2、β 和γ）、白蛋白/球蛋白比值、甘油三酯、总胆固醇、高密度脂蛋白胆固醇、极低密度脂蛋白胆固醇、低密度脂蛋白胆固醇、一氧化氮和髓过氧化物酶水平的升高。此外，HSP 和/或 TAU 的预处理或后处理也显著降低了 CCl 诱导的超氧化物歧化酶、过氧化氢酶、还原型谷胱甘肽和白蛋白浓度的降低。此外，HSP 和/或 TAU 的保护或治疗给药显著恢复了 CCl 诱导的肝组织结构改变、糖原耗竭和 DNA 含量减少。值得注意的是，保护组比治疗组更能显著减轻 CCl 诱导的肝毒性。更重要的是，在 CCl 暴露前后，HSP+TAU 的大多数生化和组织病理学参数与单独的 TAU 或 HSP 没有显著差异。总之，HSP 或 TAU 可能是 CCl 肝毒性影响的候选保护剂，但两者的联合使用仅提供有限的协同作用。图表摘要。
