Ghaith Doaa Mohammad, Zafer Mai Mahmoud, Ismail Dalia Kadry, Al-Agamy Mohamed Hamed, Bohol Marie Fe F, Al-Qahtani Ahmed, Al-Ahdal Mohammed N, Elnagdy Sherif M, Mostafa Islam Yousif
Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University, Giza, Egypt,
Infect Drug Resist. 2018 Nov 8;11:2211-2217. doi: 10.2147/IDR.S174869. eCollection 2018.
is a significant hospital-acquired pathogen, and many outbreaks of infection have been reported in neonates. We report a sudden breakout of harboring the and metallo-β-lactamase (MBL) genes that occurred from March to August 2015 in the neonatal intensive care unit of Cairo University Hospital, Cairo, Egypt.
During the study period, 40 nonduplicate clinical isolates of were collected from blood culture samples. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used to identify each isolate. Then, minimum inhibitory concentrations of different antibiotics were assessed by the Vitek 2 compact system. Screening of the MBL genes , , , , and as well as the carbapenemase genes KPC, NDM, OXA-48, SME-1, and SME-2 were evaluated. Pulsed field gel electrophoresis was preformed to detect the genetic relationship of the isolates.
Analysis showed that 37.5% of the clinical isolates were resistant to meropenem (minimum inhibitory concentrations ≥ 2 µg/mL), and and were the most prevalent MBL genes (42.5% and 37.5%, respectively). None of the other investigated genes were observed. Pulsed field gel electrophoresis typing revealed two discrete clones; 33/40 (82.5%) were pulsotype A and 7/40 (17.5%) were pulsotype B.
Here, we report for the first time the detection of MBL-producing isolates, particularly IMP-4 and VIM-2 recovered from inpatients with bacteremias from the intensive care unit at Cairo University Hospital.
是一种重要的医院获得性病原菌，新生儿中已报告多起感染暴发。我们报告了2015年3月至8月在埃及开罗开罗大学医院新生儿重症监护病房突然暴发的携带和金属β-内酰胺酶（MBL）基因的情况。
在研究期间，从血培养样本中收集了40株非重复的临床分离株。采用基质辅助激光解吸/电离飞行时间质谱法鉴定每株分离株。然后，通过Vitek 2 compact系统评估不同抗生素的最低抑菌浓度。评估了MBL基因、、、、和以及碳青霉烯酶基因KPC、NDM、OXA-48、SME-1和SME-2。进行脉冲场凝胶电泳以检测分离株的遗传关系。
分析表明，37.5%的临床分离株对美罗培南耐药（最低抑菌浓度≥2μg/mL），且和是最常见的MBL基因（分别为42.5%和37.5%）。未观察到其他研究的基因。脉冲场凝胶电泳分型显示两个不同的克隆；33/40（82.5%）为脉冲型A，7/40（17.5%）为脉冲型B。
在此，我们首次报告了从开罗大学医院重症监护病房住院菌血症患者中分离出的产MBL菌株，特别是IMP-4和VIM-2的检测情况。
