Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University, 4th Industrial Zone, Banks Complex، 6th of October, Giza, Egypt.
Department of Pharmaceutics and Microbiology, College of Pharmacy, King Saud University, 11451, Riyadh, Saudi Arabia.
BMC Infect Dis. 2015 Mar 12;15:122. doi: 10.1186/s12879-015-0861-8.
Pseudomonas aeruginosa is an important nosocomial pathogen, commonly causing infections in immunocompromised patients. The aim of this study was to examine the genetic relatedness of metallo-beta-lactamase (MBL) producing carbapenem resistant Pseudomonas aeruginosa clinical isolates collected from 2 tertiary hospitals in Cairo, Egypt using Multi Locus sequence typing (MLST).
Phenotypic and genotypic detection of metallo-beta-lactamase for forty eight non-duplicate carbapenem resistant P. aeruginosa isolates were carried out. DNA sequencing and MLST were done.
The bla VIM-2 gene was highly prevalent (28/33 strains, 85%) among 33 MBL-positive P.aeruginosa isolates. MLST revealed eleven distinct Sequence Types (STs). A unique ST233 clone producing VIM-2 was documented by MLST in P.aeruginosa strains isolated from Cairo university hospitals. The high prevalence of VIM-2 producers was not due to the spread of a single clone.
The findings of the present study clearly demonstrate that clones of VIM-2 positive in our hospitals are different from those reported from European studies. Prevalence of VIM-2 producers of the same clone was detected from surgical specimens whereas oncology related specimens were showing diverse clones.
铜绿假单胞菌是一种重要的医院获得性病原体，常导致免疫功能低下患者发生感染。本研究旨在通过多位点序列分型（MLST）方法，研究来自埃及开罗 2 家 3 级医院的 48 株非重复耐碳青霉烯类铜绿假单胞菌临床分离株中产金属β-内酰胺酶（MBL）的碳青霉烯类耐药铜绿假单胞菌的遗传相关性。
对 48 株非重复耐碳青霉烯类铜绿假单胞菌进行表型和基因型金属β-内酰胺酶检测。进行 DNA 测序和 MLST。
33 株 MBL 阳性铜绿假单胞菌中有 28 株（85%）携带 bla VIM-2 基因。MLST 显示 11 种不同的序列型（ST）。通过 MLST 在开罗大学医院分离的铜绿假单胞菌菌株中发现了一个独特的产生 VIM-2 的 ST233 克隆。VIM-2 产生菌的高流行率并非由于单个克隆的传播。
本研究结果清楚地表明，我们医院中 VIM-2 阳性的克隆与欧洲研究报道的不同。在外科标本中检测到相同克隆的 VIM-2 产生菌的流行，而肿瘤学相关标本显示出不同的克隆。
