Sereika Mantas, Urbanaviciute Ruta, Tamasauskas Arimantas, Skiriute Daina, Vaitkiene Paulina
Laboratory of Molecular Neurooncology, Neuroscience Institute, Medical Academy, Lithuanian University of Health Sciences, Eiveniu str. 4, Kaunas, LT 50009, Lithuania.
J Cancer. 2018 Oct 31;9(23):4496-4502. doi: 10.7150/jca.26769. eCollection 2018.
Glial fibrillary acidic protein (GFAP) is an intermediate filament that provides mechanical support to astrocytes. is a single nucleotide polymorphism (SNP) located in the promoter region of the gene. The aim of this pilot study is to investigate expression at mRNA, protein levels and polymorphism in 50 different grade human astrocytoma samples. expression at mRNA level was measured using quantitative reverse transcription polymerase chain reaction (qRT-PCR) with SYBR Green dye, whereas the translational activity of the following gene was detected using western blot assay. Furthermore, genotypes of were identified using qPCR with TaqMan probes. As a result, mRNA and protein expression was found to be declining with increasing astrocytoma grade (p < 0.05). A tendency was observed between increased mRNA expression and shorter grade IV astrocytoma patient survival (p = 0.2117). The CC genotype was found to be associated with increased translational activity in grade II astrocytoma (p = 0.0238). Possible links between genotypes and alternative splicing of were also observed. The AA genotype was found to be associated with poor clinical outcome for grade IV astrocytoma patients (p = 0.0007), although the following data should be checked in a larger sample size of astrocytoma patients.
胶质纤维酸性蛋白（GFAP）是一种为星形胶质细胞提供机械支撑的中间丝。[某个基因名称]是位于该基因启动子区域的一个单核苷酸多态性（SNP）。本初步研究的目的是调查50例不同分级的人类星形细胞瘤样本中[某个基因名称]在mRNA、蛋白质水平的表达以及该基因的多态性。使用带有SYBR Green染料的定量逆转录聚合酶链反应（qRT-PCR）测量mRNA水平的[某个基因名称]表达，而通过蛋白质印迹分析检测该基因的翻译活性。此外，使用带有TaqMan探针的qPCR鉴定[某个基因名称]的基因型。结果发现，随着星形细胞瘤分级增加，[某个基因名称]的mRNA和蛋白质表达下降（p < 0.05）。在IV级星形细胞瘤患者中观察到[某个基因名称]mRNA表达增加与较短生存期之间存在一种趋势（p = 0.2117）。发现[某个基因名称]的CC基因型与II级星形细胞瘤中[某个基因名称]的翻译活性增加相关（p = 0.0238）。还观察到[某个基因名称]基因型与[某个基因名称]的可变剪接之间可能存在联系。发现[某个基因名称]的AA基因型与IV级星形细胞瘤患者的不良临床结局相关（p = 0.0007），尽管应在更大样本量的星形细胞瘤患者中检查以下数据。
