Sethabutr O, Brown A E, Gingrich J, Webster H K, Pooyindee N, Taylor D N, Echeverria P
Department of Bacteriology, Armed Forces Research Institute of Medical Sciences, Bankok, Thailand.
Am J Trop Med Hyg. 1988 Sep;39(3):227-31. doi: 10.4269/ajtmh.1988.39.227.
Synthetic, 21-base, DNA probes to the genome of Plasmodium falciparum were either 32P-labeled or enzyme-conjugated for comparative field studies. The sensitivity of both probes was compared with microscopy in the examination of blood samples from 97 Thai villagers, 47 Thai Rangers, and 19 malaria-free Bangkok residents. The probes were also used to monitor the therapeutic response of 18 of the Rangers during 7 days of treatment. The probes proved highly specific. Both probes had lower limits of detection of about 100 parasites per microliter blood. Thus, the low parasite densities in partially immune villagers from an endemic area were often missed, while higher parasite densities in the nonimmune Rangers were usually detected. As monitors of response to treatment, the probes paralleled microscopy in identifying reversion from positive to negative parasitemia. The enzymelabeled DNA probe as shown to perform similarly to the radiolabeled probe in populations with different malarial immune status and during curative treatment.
针对恶性疟原虫基因组的合成21碱基DNA探针，分别用32P标记或酶标记，用于比较性现场研究。在对97名泰国村民、47名泰国护林员和19名无疟疾的曼谷居民的血样检测中，将两种探针的灵敏度与显微镜检查进行了比较。这些探针还用于监测18名护林员在7天治疗期间的治疗反应。结果证明这些探针具有高度特异性。两种探针的检测下限均约为每微升血液100个寄生虫。因此，来自流行地区的部分免疫村民体内低寄生虫密度常常漏检，而非免疫的护林员体内较高的寄生虫密度通常能被检测到。作为治疗反应的监测手段，在确定寄生虫血症从阳性转为阴性方面，探针与显微镜检查结果相似。在不同疟疾免疫状态的人群以及治疗过程中，酶标记DNA探针表现与放射性标记探针相似。
