a Department of Zoology , University of Johannesburg , Johannesburg , South Africa.
b Department of Zoology , University of Venda , Thohoyandou , South Africa.
Int J Environ Health Res. 2019 Aug;29(4):441-456. doi: 10.1080/09603123.2018.1548697. Epub 2018 Dec 6.
The anti-retroviral nevirapine has been detected in surface waters throughout South Africa and its effects on non-target aquatic animals are still unknown. The aim was to investigate the potential effects of nevirapine on the hatching success and survival of early life stages through a chronic exposure. The exposer started with newly fertilized eggs and concluded 30 days after hatching. Environmental relevant concentration of nevirapine (1.48 µg/l) was used in a static renewal system and a controlled environment (27 ± 1°C; 14:10 day/night cycle). The main endpoints assessed included hatching success and survival; a morphological assessment was also done on whole individual on day 1 and 30 post-hatching to identify any physical abnormality. Nevirapine had no noticeable effects on the hatching success and survival of larvae; no statistically significant differences were observed between the control and the nevirapine exposed fish ( > 0.05).
抗逆转录病毒药物奈韦拉平已在南非地表水检测到，但其对非目标水生动物的影响尚不清楚。本研究旨在通过慢性暴露，调查奈韦拉平对早期生命阶段孵化成功率和生存的潜在影响。暴露始于新受精的卵，持续 30 天直至孵化结束。实验采用静态更新系统和控制环境（27±1°C；14:10 昼/夜循环），使用环境相关浓度的奈韦拉平（1.48μg/l）。评估的主要终点包括孵化成功率和存活率；孵化后第 1 天和第 30 天还对完整个体进行了形态评估，以确定任何身体异常。奈韦拉平对幼虫的孵化成功率和存活率没有明显影响；在对照组和奈韦拉平暴露组之间未观察到统计学上的显著差异（>0.05）。
