Ohrloff C, Stoffel C, Koch H R, Wefers U, Bours J, Hockwin O
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1978 Jan 31;205(2):73-9. doi: 10.1007/BF00410102.
A tryptophan-free diet induces posterior subcapsular cataracts and reversible corneal opacities in young Wistar rats. Compared to the controls there is a significant decrease of body weight, lens fresh weight, and water-soluble lens protein. Protein separation by isoelectric focusing shows diminished alpha-, beta-, and gamma-crystallin fractions. If tryptophan is restored to the diet after 24 days, new clear lens fibers are laid down again, indicating that the mechanism of protein synthesis has not been permanently damaged by tryptophan-deficiency.
无色氨酸饮食可诱导幼年Wistar大鼠发生后囊下白内障和可逆性角膜混浊。与对照组相比，体重、晶状体鲜重和水溶性晶状体蛋白显著降低。通过等电聚焦进行蛋白质分离显示α-、β-和γ-晶状体蛋白组分减少。如果在24天后饮食中恢复色氨酸，新的透明晶状体纤维会再次形成，这表明蛋白质合成机制并未因色氨酸缺乏而受到永久性损害。
