Wegener A, Golubnitschaja O, Breipohl W, Schild H H, Vrensen G F J M
Department of Experimental Ophthalmology, Medical Faculty, Rheinische Friedrich-Wilhelms-Universität, Bonn, Germany.
Amino Acids. 2002;23(1-3):337-42. doi: 10.1007/s00726-001-0147-x.
Effects of dietary deficiencies of tryptophan and methionin on the transparency of cornea and lens were investigated in young rats (Brown-Norway, BN; Sprague-Dawley, SD) over 3 months. Transparency of the cornea and lens were evaluated in weekly intervals using a photo-slitlamp microscope. After sacrifice and lens fresh weight determination the lenses were prepared for histopathology. Methionin deficiency had no effect on the parameters investigated. Tryptophan deficiency caused severe loss of body weight in both strains, with additional loss of hair in SD rats. These developed corneal neovascularisations and cataracts. BN rats showed an enhanced zone of discontinuity in the lens. Diet intermission arrested the pathological processes in the eye which restarted when feeding the diet again. This observation is supported by lens fresh weight data. DNA staining evidenced that tryptophan deficiency arrested lens fiber maturation in both strains but stimulated corneal neovascularisation only in SD rats.
在3个月以上的幼鼠（布朗-挪威大鼠，BN；斯普拉格-道利大鼠，SD）中研究了色氨酸和蛋氨酸饮食缺乏对角膜和晶状体透明度的影响。使用光裂隙灯显微镜每周评估角膜和晶状体的透明度。在处死后测定晶状体鲜重，然后将晶状体制备用于组织病理学检查。蛋氨酸缺乏对所研究的参数没有影响。色氨酸缺乏导致两种品系的大鼠体重严重减轻，SD大鼠还出现额外的毛发脱落。这些大鼠出现了角膜新生血管和白内障。BN大鼠晶状体中的间断区增强。饮食中断阻止了眼部的病理过程，再次喂食该饮食时病理过程重新开始。晶状体鲜重数据支持了这一观察结果。DNA染色证明，色氨酸缺乏在两种品系中均阻止了晶状体纤维成熟，但仅在SD大鼠中刺激了角膜新生血管形成。
