Department of Chemistry, Payame Noor University (PNU), P.O. Box: 19395-4697, Tehran, Iran.
Colloids Surf B Biointerfaces. 2019 Mar 1;175:98-105. doi: 10.1016/j.colsurfb.2018.11.087. Epub 2018 Dec 1.
A simple, low-cost and sensitive label-free aptasensor assembled with assisting reduced graphene oxide nanosheets as the signal amplifier was fabricated and applied for detecting ultra-low levels of Aflatoxin B1(AFB1) through a nano-bio interaction system. The conditions of different modified glassy carbon electrodes as the base of aptasensor were investigated by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The performance of the fabricated aptasensor was evaluated by FESEM, HRTEM and AFM images. The proposed biosensor detected AFB1sensitively in a wide linear range (0.5 nM-4μM) by DPV with a considerable low limit of detection (LOD = 0.07 nM) and good repeatability (RSD = 2.9) and stability. Finally, the present aptasensor was applied successfully for monitoring AFB1 with appropriate recoveries in pasteurized cow milk and human blood plasma as real samples.
一种简单、低成本且灵敏的无标记适体传感器，采用辅助还原氧化石墨烯纳米片作为信号放大器，通过纳米生物相互作用系统制备并用于检测极低水平的黄曲霉毒素 B1（AFB1）。通过循环伏安法（CV）、差分脉冲伏安法（DPV）和电化学阻抗谱（EIS）研究了不同修饰玻碳电极作为适体传感器基底的条件。通过 FESEM、HRTEM 和 AFM 图像评估了所制备的适体传感器的性能。该生物传感器通过 DPV 在较宽的线性范围内（0.5 nM-4 μM）对 AFB1 进行了灵敏检测，具有相当低的检测限（LOD=0.07 nM）和良好的重复性（RSD=2.9%）和稳定性。最后，该适体传感器成功应用于巴氏杀菌牛奶和人血浆等实际样品中 AFB1 的监测，回收率适宜。
