Masood S
University of Florida College of Medicine, Jacksonville.
Cancer Detect Prev. 1988;12(1-6):283-90.
Ovarian cancer specimens from 45 patients were assayed for the presence of estrogen receptor (ER) utilizing highly specific monoclonal antiestrophilin antibodies and the peroxidase-antiperoxidase technique. Results were compared with the conventional ER determination of the dextran charcoal method (DCC) and were in concordance in 91% of cases. This technique was also used to analyze ER in fine needle aspiration biopsy specimen from four patients with metastatic ovarian tumor. These results suggest that antireceptor monoclonal antibody in immunohistochemical analysis is an effective tool in the evaluation of estrogen receptor content in ovarian cancer. This technique can be easily performed at community hospitals and may be extended to the evaluation of fine needle aspiration biopsies and to analyze specimens of insufficient size for biochemical assay.
利用高度特异性的抗雌激素受体蛋白单克隆抗体和过氧化物酶-抗过氧化物酶技术，对45例患者的卵巢癌标本进行雌激素受体（ER）检测。将结果与葡聚糖包被活性炭法（DCC）的传统ER测定结果进行比较，91%的病例结果一致。该技术还用于分析4例转移性卵巢肿瘤患者细针穿刺活检标本中的ER。这些结果表明，免疫组织化学分析中的抗受体单克隆抗体是评估卵巢癌雌激素受体含量的有效工具。该技术在社区医院易于操作，可扩展用于细针穿刺活检评估以及分析生化检测样本量不足的标本。
