Azavedo E, Baral E, Skoog L
Anticancer Res. 1986 Mar-Apr;6(2):263-6.
An immunocytochemical assay for the estrogen receptor has been used to analyze the receptor content in tumor cells obtained by fine needle aspiration from human breast carcinomas. The results were compared with receptor determinations on cryostat sections of freshly frozen tumors and by biochemical technique based on radiolabelled ligand binding. The staining intensity and fraction of positive cells were comparable in cryostat sections and tumor cell specimens obtained by fine needle aspiration. Moreover, there was a good correlation between the semiquantitative receptor level estimated by the immunoperoxidase technique and the biochemical assay. These results show that tumor cell specimens obtained by fine needle aspirates can be used for estrogen receptor analysis by the immunocytochemical technique.
一种雌激素受体免疫细胞化学检测方法已被用于分析通过细针穿刺从人乳腺癌获取的肿瘤细胞中的受体含量。将结果与新鲜冷冻肿瘤的低温恒温器切片上的受体测定结果以及基于放射性标记配体结合的生化技术测定结果进行比较。低温恒温器切片和通过细针穿刺获得的肿瘤细胞标本中的染色强度和阳性细胞比例相当。此外，通过免疫过氧化物酶技术估计的半定量受体水平与生化检测之间存在良好的相关性。这些结果表明，通过细针穿刺获得的肿瘤细胞标本可用于免疫细胞化学技术的雌激素受体分析。
