School of Medical Sciences and Kirby Institute for Infection and Immunity, UNSW Australia, 2052, NSW, Australia.
School of Medical Sciences and Kirby Institute for Infection and Immunity, UNSW Australia, 2052, NSW, Australia.
Biochim Biophys Acta Gen Subj. 2019 Feb;1863(2):511-519. doi: 10.1016/j.bbagen.2018.12.003. Epub 2018 Dec 6.
Next generation sequencing (NGS) methods have significantly contributed to a paradigm shift in genomic research for nearly a decade now. These methods have been useful in studying the dynamic interactions between RNA viruses and human hosts.
In this review, we summarise and discuss key applications of NGS in studying the host - pathogen interactions in RNA viral infections of humans with examples.
Use of NGS to study globally relevant RNA viral infections have revolutionized our understanding of the within host and between host evolution of these viruses. These methods have also been useful in clinical decision-making and in guiding biomedical research on vaccine design.
NGS has been instrumental in viral genomic studies in resolving within-host viral genomic variants and the distribution of nucleotide polymorphisms along the full-length of viral genomes in a high throughput, cost effective manner. In the future, novel advances such as long read, single molecule sequencing of viral genomes and simultaneous sequencing of host and pathogens may become the standard of practice in research and clinical settings. This will also bring on new challenges in big data analysis.
下一代测序（NGS）方法在近十年的基因组研究中引发了重大变革。这些方法在研究 RNA 病毒与人类宿主之间的动态相互作用方面非常有用。
在本文中，我们总结并讨论了 NGS 在研究人类 RNA 病毒感染中宿主-病原体相互作用的关键应用，同时举例说明。
使用 NGS 研究具有全球相关性的 RNA 病毒感染，彻底改变了我们对这些病毒在宿主内和宿主间进化的理解。这些方法还可用于临床决策制定和指导疫苗设计的生物医学研究。
NGS 在解决病毒基因组内的变异和核苷酸多态性分布方面的病毒基因组研究中具有重要意义，可实现高通量、具有成本效益的方式对全长病毒基因组进行测序。未来，长读长、病毒基因组单分子测序以及宿主和病原体的同时测序等新进展可能成为研究和临床环境中的标准实践，这也将给大数据分析带来新的挑战。
