Departments of Pediatrics and Microbiology, New York University School of Medicine, New York, NY, USA.
Departments of Pediatrics and Microbiology, New York University School of Medicine, New York, NY, USA.
Vaccine. 2019 Jan 14;37(3):409-411. doi: 10.1016/j.vaccine.2018.12.001. Epub 2018 Dec 8.
Group B Streptococcus (GBS) is a neonatal pathogen frequently transmitted from maternal asymptomatic vagino-rectal colonization. Co-colonization with multiple GBS serotypes, which has implications for type-specific vaccination strategies, is difficult to detect with standard microbiologic techniques. We designed a nested real-time PCR assay to detect vaginal co-colonization in samples from a cohort of non-pregnant women (N = 433). 6/91 (6.6%) GBS-positive samples harbored ≥2 GBS serotypes, with over-representation of serotype V among co-colonized samples. Serotype IV GBS was more prevalent (>10%) in this cohort than in previously reported United States studies. Ongoing surveillance of GBS serotype epidemiology and co-colonization is indicated.
无乳链球菌（GBS）是一种新生儿病原体，常通过母体无症状的阴道直肠定植传播。用标准微生物学技术很难检测到与多种 GBS 血清型共同定植的情况，而这对特定类型的疫苗接种策略有影响。我们设计了一种巢式实时 PCR 检测方法，用于检测非孕妇队列样本中的阴道共同定植情况（N=433）。91 个 GBS 阳性样本中有 6 个（6.6%）携带≥2 种 GBS 血清型，共同定植样本中血清型 V 占比过高。本研究中血清型 IV GBS 的流行率（>10%）高于之前在美国的报道。需要对 GBS 血清型流行病学和共同定植情况进行持续监测。
