Department of Food Science and Nutrition, Zhejiang University, Hangzhou, 310058, PR China; Zhejiang Key Laboratory for Agro-food Processing, Zhejiang University, Hangzhou, 310058, PR China; Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, PR China.
Division of Toxicology, Wageningen University, Stippeneng 4, 6708, WE, Wageningen, the Netherlands.
Food Chem Toxicol. 2019 Feb;124:231-238. doi: 10.1016/j.fct.2018.12.003. Epub 2018 Dec 7.
Since flavonoids are antioxidant compounds, they could beneficially affect neurodegenerative diseases where reactive oxygen species are involved. In this study, we firstly isolated and identified fourteen compounds from the flowers of Apios americana Medik. Then, we tested whether Apios americana Medik flowers water extract (AFWE) exerts a protective effect on HO induced PC12 cells injure. As expected, pretreatment with AFWE inhibited cytotoxicity and DNA condensation in HOinduced PC12 cells. Exposure of PC12 cells to HO resulted in reactive oxygen species accumulation and mitochondrial dysfunction, while AFWE alleviated these damages. AFWE obviously reversed the alternations as H2O2 increased Caspase-3 and decreased the ratio of Bcl-2/Bax expressions. Furthermore, autophagy in PC12 cells was further activated by AFWE, which was beneficial to resisting adversity. These results manifest that AFWE prevents HO induced damage via regulating autophagy.
由于类黄酮是抗氧化化合物，因此它们可能对涉及活性氧的神经退行性疾病有益。在这项研究中，我们首先从美洲菜豆的花中分离和鉴定了 14 种化合物。然后，我们测试了美洲菜豆花水提取物（AFWE）是否对 HO 诱导的 PC12 细胞损伤发挥保护作用。正如预期的那样，AFWE 预处理可抑制 HO 诱导的 PC12 细胞的细胞毒性和 DNA 凝聚。PC12 细胞暴露于 HO 会导致活性氧积累和线粒体功能障碍，而 AFWE 减轻了这些损伤。AFWE 明显逆转了 H2O2 增加 Caspase-3 和降低 Bcl-2/Bax 表达比值的变化。此外，AFWE 进一步激活了 PC12 细胞中的自噬，这有利于抵抗逆境。这些结果表明，AFWE 通过调节自噬来防止 HO 诱导的损伤。
