Fu Yaling, Zhang Yanlei, Zhang Rentang
College of Food Science and Engineering Shandong Agricultural University Tai'an China.
Food Sci Nutr. 2021 Jul 23;9(9):5070-5082. doi: 10.1002/fsn3.2464. eCollection 2021 Sep.
In order to investigate the purification process and antioxidant activity of triterpenic acids from blackened jujube, the macroporous resin was applied to purify the crude extract from blackened jujube. The adsorption and desorption characterizations of five different macroporous adsorption resins (AB-8, D-101, X-5, HPD-100, S-8) for triterpenic acids of blackened jujube were compared, the optimum purification resins were screened, and the purification parameters were optimized. The antioxidant activity of crude extracts and purified products from blackened jujube was analyzed. The results showed that D-101 resin possessed the best effect on the purification of blackened jujube triterpenic acids. The optimum purification parameters were as follows: sample concentration 25.5 μg/ml, 130 ml of the sample volume was with a flow rate of 2.0 ml/min, eluted with 95% ethanol, and speed flow was 1.0 ml/min. The purity of triterpenic acids was increased by 2.49 times after purification with a recovery rate of (78.58 ± 0.67)%. Furthermore, the IC values of hydroxyl radical scavenging capacity from triterpenic acids crude extract and purified substances were 0.900 and 0.850 mg/ml, respectively, and the IC values of superoxide anion radical were 0.745 and 0.594 mg/ml, respectively, indicating that the antioxidative capacity of the purified product was stronger than the crude extract. The purified triterpenic acids (PTA) groups at different doses had excellent protective effects on HO-induced damage HUVEC cells. Results have revealed that triterpenic acids of blackened jujube have good antioxidant function and utilization and development prospects.
为研究黑枣中三萜酸的纯化工艺及抗氧化活性，采用大孔树脂对黑枣粗提物进行纯化。比较了5种不同大孔吸附树脂（AB - 8、D - 101、X - 5、HPD - 100、S - 8）对黑枣三萜酸的吸附和解吸特性，筛选出最佳纯化树脂并优化了纯化参数。分析了黑枣粗提物及纯化产物的抗氧化活性。结果表明，D - 101树脂对黑枣三萜酸的纯化效果最佳。最佳纯化参数如下：样品浓度25.5 μg/ml，进样量130 ml，流速2.0 ml/min，用95%乙醇洗脱，洗脱流速1.0 ml/min。纯化后三萜酸纯度提高了2.49倍，回收率为（78.58±0.67）%。此外，三萜酸粗提物和纯化产物对羟基自由基清除能力的IC值分别为0.900和0.850 mg/ml，对超氧阴离子自由基的IC值分别为0.745和0.594 mg/ml，表明纯化产物的抗氧化能力强于粗提物。不同剂量的纯化三萜酸（PTA）组对过氧化氢诱导的人脐静脉内皮细胞（HUVEC）损伤具有良好的保护作用。结果表明，黑枣三萜酸具有良好的抗氧化功能及利用和开发前景。
