Verme C N, Ludden T M, Harris S C
Research Service, Audie L. Murphy Memorial Veterans Hospital, San Antonio, TX 78284-7765.
Clin Chem. 1988 Nov;34(11):2316-20.
There is an increasing demand for quantification of serum alpha 1-acid glycoprotein (AAG, orosomucoid) in studies evaluating the protein binding of highly bound basic drugs. This paper describes an adaptation of an automated immunoturbidimetric assay for this protein to the Cobas Bio centrifugal analyzer. Replicate analyses of aliquots from six different solutions were used in determining precision. We also analyzed 367 patients' serum samples, in duplicate, to determine the distribution of AAG in hospitalized patients. The intra- and inter-run CVs ranged from 1.3% to 4.4% and from 0.6% to 6.6%, respectively. AAG concentrations in patients' samples ranged from 0.38 to 3.16 g/L. Results by this method correlate well with those by radial immunodiffusion, with no significant amount of bias between the two methods. This immunoturbidimetric procedure is faster and less expensive than currently used radial immunodiffusion techniques, and precision is acceptable.
在评估高结合率碱性药物的蛋白质结合情况的研究中，对血清α1-酸性糖蛋白（AAG，类粘蛋白）定量的需求日益增加。本文描述了一种针对该蛋白质的自动化免疫比浊法在Cobas Bio离心分析仪上的应用。通过对六种不同溶液的等分试样进行重复分析来确定精密度。我们还对367例患者的血清样本进行了双份分析，以确定住院患者中AAG的分布情况。批内和批间变异系数分别为1.3%至4.4%和0.6%至6.6%。患者样本中AAG浓度范围为0.38至3.16 g/L。该方法的结果与放射免疫扩散法的结果相关性良好，两种方法之间无显著偏差。这种免疫比浊法比目前使用的放射免疫扩散技术更快且成本更低，精密度也可接受。
