School of Environmental and Safety Engineering, Changzhou University, Changzhou 213164, PR China.
School of Environmental and Safety Engineering, Changzhou University, Changzhou 213164, PR China.
Ecotoxicol Environ Saf. 2019 Apr 15;170:218-226. doi: 10.1016/j.ecoenv.2018.11.136. Epub 2018 Dec 6.
To understand the importance of the response of soil microbial communities to the stress of heavy metals around mining areas by assessing the feedback of soil ecosystems in different soil habitats, this article selected different land use types (Mining area, Dressing area, Heap mine area, Tailings area and Vegetable field) and surface soil samples of different depths (0-10 cm, 10-20 cm, 20-30 cm) as the variables related to the mining activities in the Shizishan mining area in Tongling, Anhui Province, China. Soil physicochemical properties and heavy metal concentrations of the different land use types and soil depths were compared. Illumina MiSeq. 2500 Sequencing Technology was used to analyze the abundance and structural diversity of the microbial community in soil samples. The relationship between mine soil pollution characteristics and microbial community were investigated. The results showed that soil physicochemical properties and heavy metals significantly affected the microbial community. The microbial community structure was significantly variable in vertical soil depth-layer habitats. The relative abundance (1%) of the soil microbial community at the phylum level was represented by a total of 14 phyla, where the two most dominant phyla were Proteobacteria (41.71%) and Firmicutes (20.44%). The two bacteria were positively related with Cu, Zn, Pb, and pH but negatively associated with soil organic matter (SOM), available potassium (AK), and moisture content (MC). Therefore, Proteobacteria and Firmicutes were highly resistant to heavy metals. These results increased our understanding of microbial variation and assembly pattern under different land use types in heavy metals contaminated mining soils.
为了了解矿区周围土壤微生物群落对重金属胁迫的响应的重要性，通过评估不同土壤生境中土壤生态系统的反馈，本文选择了不同的土地利用类型（矿区、选矿区、排土场、尾矿库和菜地）和不同深度的表层土壤样本（0-10cm、10-20cm、20-30cm）作为与安徽省铜陵市狮子山矿区采矿活动相关的变量。比较了不同土地利用类型和土壤深度的土壤理化性质和重金属浓度。采用 Illumina MiSeq.2500 测序技术分析土壤样本中微生物群落的丰度和结构多样性。研究了矿区土壤污染特征与微生物群落的关系。结果表明，土壤理化性质和重金属显著影响了微生物群落。垂直土壤深度层生境中的微生物群落结构存在显著差异。土壤微生物群落的相对丰度（1%）在门水平上共代表了 14 个门，其中最主要的两个门是变形菌门（41.71%）和厚壁菌门（20.44%）。这两种细菌与 Cu、Zn、Pb 和 pH 呈正相关，与土壤有机质（SOM）、有效钾（AK）和含水量（MC）呈负相关。因此，变形菌门和厚壁菌门对重金属具有很强的抵抗力。这些结果增加了我们对重金属污染矿区不同土地利用类型下微生物变异和组装模式的理解。
