含纳米羟磷灰石脱敏牙膏对牙本质小管封闭能力和自酸蚀粘结剂粘结性能的影响。

Influence of nano-hydroxyapatite containing desensitizing toothpastes on the sealing ability of dentinal tubules and bonding performance of self-etch adhesives.

机构信息

Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi'an Jiaotong University, 710004 Xi'an, China; Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, 710004 Xi'an, China; Department of Prosthodontics, College of Stomatology, Xi'an Jiaotong University, 710004 Xi'an, China.

Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi'an Jiaotong University, 710004 Xi'an, China.

出版信息

J Mech Behav Biomed Mater. 2019 Mar;91:38-44. doi: 10.1016/j.jmbbm.2018.11.021. Epub 2018 Nov 28.

DOI:10.1016/j.jmbbm.2018.11.021

PMID:30529985

Abstract

OBJECTIVES

To evaluate the dentinal tubular occlusion of nano-hydroxyapatite (nHAp) containing desensitizing toothpastes and their influence on the resin-dentin bonding performance of two mild self-etch adhesives.

MATERIALS AND METHODS

Mid-coronal dentin specimens were prepared from obtained intact human third molars. They were immersed in 1% citric acid for 20 s to expose the dentinal tubules to simulate sensitive teeth and then randomly divided into four groups. The control group received no desensitizing treatment. Experimental groups were treated with two commercial nHAp containing desensitizing toothpastes (Biorepair and Dontodent) and an experimental pure nHAp paste respectively. Each group was further divided into two subgroups and bonded with either G-Bond or Clearfil S Bond. The micro-tensile bond strength was tested and failure mode distribution was analyzed. Moreover, the effect of desensitizers on dentinal tubular occlusion was observed by the field-emission scanning electron microscope (FESEM). Resin infiltration of the adhesives labeled by fluorescent Rhodamin B was evaluated under confocal laser scanning microscopy (CLSM).

RESULTS

FESEM revealed that all the desensitizers noticeably occluded the dentinal tubules, and the extents were completer after application for 7 days. The majority of the occlusion still preserved even after acid challenge with cola or adhesive. CLSM demonstrated shorter resin tags were produced in the desensitized groups. When bonding with G-Bond, the pure nHAp group showed comparable bond strength to the control group, while Biorepair and Dontodent treatment decreased the bond strength. For groups bonded with Clearfil S Bond, all the desensitizers reduced the bond strengths compared to the control and no significant difference was found among the three groups.

CONCLUSION

Nano-hydroxyapatite containing desensitizing toothpastes could occlude dentinal tubules effectively with a certain degree of acid resistance, which contributes to the relief of dentin hypersensitivity. While, the application of these nHAp desensitizers comprised the resin infiltration of G-Bond and Clearfil S Bond, resulting in decreased bond strengths of the resin-dentin bonding.

摘要

目的

评估含有脱敏剂的纳米羟基磷灰石（nHAp）对牙本质小管的封闭作用及其对两种温和自酸蚀粘结剂的树脂-牙本质粘结性能的影响。

材料和方法

从获得的完整人第三磨牙中制备近中牙本质标本。将它们浸入 1%柠檬酸中 20s，以暴露牙本质小管，模拟敏感牙齿，然后将其随机分为四组。对照组未进行脱敏处理。实验组分别用两种市售含 nHAp 的脱敏牙膏（Biorepair 和 Dontodent）和一种实验性纯 nHAp 糊剂处理。每组进一步分为两个亚组，分别用 G-Bond 或 Clearfil S Bond 粘结。测试微拉伸粘结强度，并分析失效模式分布。此外，通过场发射扫描电子显微镜（FESEM）观察脱敏剂对牙本质小管的封闭效果。通过共聚焦激光扫描显微镜（CLSM）评估荧光罗丹明 B 标记的粘结剂的树脂渗透情况。

结果

FESEM 显示所有脱敏剂均明显封闭牙本质小管，7 天后封闭程度更完全。即使在可乐或粘结剂的酸蚀后，大多数封闭仍然保留。CLSM 显示脱敏组产生的树脂标记更短。用 G-Bond 粘结时，纯 nHAp 组的粘结强度与对照组相当，而 Biorepair 和 Dontodent 处理则降低了粘结强度。用 Clearfil S Bond 粘结的各组与对照组相比，所有脱敏剂均降低了粘结强度，但三组之间无显著差异。