用于快速检测多重耐药菌株的结晶紫脱色试验：一项多中心研究。

Crystal violet decolorization assay for rapid detection of multidrug-resistant isolates: A multicenter study.

作者信息

Coban Ahmet Yilmaz, Alakbarova Gumral, Satti Luqman, Tayyab Nadia, Zaman Gohar, Morcillo Nora, Imperiale Belen, Wainmayer Ingrid, Simboli Norberto

机构信息

Department of Nutrition and Dietetics, Faculty of Health Sciences, Akdeniz University, Antalya, Turkey.

Thalassemia Center, Medical Microbiology Laboratory, AZ1072 Baku, Azerbaijan.

出版信息

Int J Mycobacteriol. 2018 Oct-Dec;7(4):310-314. doi: 10.4103/ijmy.ijmy_141_18.

DOI:10.4103/ijmy.ijmy_141_18

PMID:30531027

Abstract

BACKGROUND

Effective control of tuberculosis is achieved by early diagnosis and drug susceptibility testing for initiation of appropriate treatment. The performance of crystal violet decolorization assay (CVDA) for susceptibility testing of Mycobacterium tuberculosis to isoniazid (INH) and rifampicin (RIF) was compared in a multicenter study.

METHODS

Seventy-two M. tuberculosis isolates were tested in two phases by CVDA.

RESULTS

In Phase I, the specificity, sensitivity, positive predictive value (PPV), negative predictive value (NPV), and agreement for INH were 100%, respectively. Specificity, sensitivity, PPV, NPV, and agreement for RIF were 98.2%, 100%, 94.1%, 100%, and 98.6%, respectively. In Phase II, specificity, sensitivity, PPV, NPV, and agreement were 98%, 100%, 95.4%, 100%, and 98.6% for INH, respectively. Specificity, sensitivity, PPV, NPV, and agreement for RIF were 96.3%, 88.2%, 88.2%, 96.3%, and 94.4%, respectively. Results in the study were obtained on average 10.9 ± 3.1 days in Phase I and 9.8 ± 2.2 days in Phase II.

CONCLUSION

CVDA can be performed for drug susceptibility testing in developed and developing countries. In addition, further studies with larger sample size are needed for evaluation of this method.

摘要

背景

通过早期诊断和药物敏感性测试以启动适当治疗来实现结核病的有效控制。在一项多中心研究中，对结晶紫脱色试验（CVDA）用于结核分枝杆菌对异烟肼（INH）和利福平（RIF）敏感性测试的性能进行了比较。

方法

通过CVDA分两个阶段对72株结核分枝杆菌分离株进行测试。

结果

在第一阶段，INH的特异性、敏感性、阳性预测值（PPV）、阴性预测值（NPV）和一致性分别为100%。RIF的特异性、敏感性、PPV、NPV和一致性分别为98.2%、100%、94.1%、100%和98.6%。在第二阶段，INH的特异性、敏感性、PPV、NPV和一致性分别为98%、100%、95.4%、100%和98.6%。RIF的特异性、敏感性、PPV、NPV和一致性分别为96.3%、88.2%、88.2%、96.3%和94.4%。研究结果在第一阶段平均10.9±3.1天获得，在第二阶段平均9.8±2.2天获得。