Davison Gareth, Vinaixa Maria, McGovern Rose, Beltran Antoni, Novials Anna, Correig Xavier, McClean Conor
Sport and Exercise Science Research Institute, Ulster University, Antrim, United Kingdom.
Metabolomics Platform of the Spanish Biomedical Research Center in Diabetes and Associated Metabolic Disorders, IISPV - Rovira i Virgili University, Tarragona, Spain.
Front Physiol. 2018 Nov 26;9:1682. doi: 10.3389/fphys.2018.01682. eCollection 2018.
Metabolomics is a relatively new "omics" approach used to characterize metabolites in a biological system at baseline and following a diversity of stimuli. However, the metabolomic response to exercise in hypoxia currently remains unknown. To examine this, 24 male participants completed 1 h of exercise at a workload corresponding to 75% of pre-determined O in hypoxia (Fo = 0.16%), and repeated in normoxia (Fo = 0.21%), while pre- and post-exercise and 3 h post-exercise metabolites were analyzed using a LC ESI-qTOF-MS untargeted metabolomics approach in serum samples. Exercise in hypoxia and in normoxia independently increased metabolism as shown by a change in a combination of twenty-two metabolites associated with lipid metabolism ( < 0.05, pre vs. post-exercise), though hypoxia did not induce a greater metabolic change when compared with normoxia ( > 0.05). Recovery from exercise in hypoxia independently decreased seventeen metabolites associated with lipid metabolism ( < 0.05, post vs. 3 h post-exercise), compared with twenty-two metabolites in normoxia ( < 0.05, post vs. 3 h post-exercise). Twenty-six metabolites were identified as responders to exercise and recovery (pooled hypoxia and normoxia pre vs. recovery, < 0.05), including metabolites associated with purine metabolism (adenine, adenosine and hypoxanthine), the amino acid phenylalanine, and several acylcarnitine molecules. Our novel data provides preliminary evidence of subtle metabolic differences to exercise and recovery in hypoxia and normoxia. Specifically, exercise in hypoxia activates metabolic pathways aligned to purine and lipid metabolism, but this effect is not selectively different from exercise in normoxia. We also show that exercise can activate pathways associated with lipid, protein and purine nucleotide metabolism.
代谢组学是一种相对较新的“组学”方法，用于在基线状态以及受到多种刺激后对生物系统中的代谢物进行表征。然而，目前尚不清楚低氧环境下运动的代谢组学反应。为了对此进行研究，24名男性参与者在低氧环境（Fₒ = 0.16%）下以相当于预先确定的摄氧量75%的工作量完成了1小时的运动，并在常氧环境（Fₒ = 0.21%）下重复进行，同时在运动前、运动后以及运动后3小时，使用液相色谱-电喷雾电离-四极杆飞行时间质谱非靶向代谢组学方法对血清样本中的代谢物进行分析。低氧环境和常氧环境下的运动均独立地增加了代谢，这表现为与脂质代谢相关的22种代谢物组合发生了变化（P < 0.05，运动前与运动后），不过与常氧环境相比，低氧环境并未诱导出更大的代谢变化（P > 0.05）。低氧环境下运动后的恢复独立地降低了17种与脂质代谢相关的代谢物（P < 0.05，运动后与运动后3小时），而常氧环境下为22种代谢物（P < 0.05，运动后与运动后3小时）。26种代谢物被确定为对运动和恢复有反应（低氧和常氧合并的运动前与恢复后，P < 0.05），包括与嘌呤代谢相关的代谢物（腺嘌呤、腺苷和次黄嘌呤）、氨基酸苯丙氨酸以及几种酰基肉碱分子。我们的新数据提供了初步证据，表明低氧和常氧环境下运动及恢复存在细微的代谢差异。具体而言，低氧环境下的运动激活了与嘌呤和脂质代谢相关的代谢途径，但这种效应与常氧环境下的运动并无选择性差异。我们还表明，运动可以激活与脂质、蛋白质和嘌呤核苷酸代谢相关的途径。
