Souza Vanessa R, Mendes Eduardo, Casaro Mateus, Antiorio Ana Tada F B, Oliveira Fernando A, Ferreira Caroline M
Department of Pharmaceutical Sciences, Institute of Environmental, Chemistry and Pharmaceutical Sciences, Universidade Federal de São Paulo, Diadema, SP, Brazil.
Department of Pathology, Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil.
Methods Mol Biol. 2019;1916:303-309. doi: 10.1007/978-1-4939-8994-2_29.
Estrogen and ovarian function decline are relevant characteristics of menopause period. Numerous physiological, metabolic and immunological alterations in the female body occur in the menopause period and some of these changes remain uncertain. The animal model that mimics menopause phase is an important approach to better comprehend the biological process involved in this period of women life. Ovariectomy is a procedure where ovaries are surgically excised and have been a valuable tool for understanding estrogen deficiency through animal experiments. Despite the diversity of ovariectomy protocols, the aim of this chapter is to provide a comprehensive guideline in performing ovariectomy in mice. Furthermore, isoflurane anesthesia system, postoperative care and surgery success evaluation will be described. We highlight that all procedures must be carried out by a qualified and trained professional, respecting ethical and safety principles.
雌激素和卵巢功能衰退是更年期的相关特征。女性身体在更年期会出现许多生理、代谢和免疫方面的改变，其中一些变化仍不明确。模拟更年期阶段的动物模型是更好理解女性生命这一时期所涉及生物学过程的重要方法。卵巢切除术是一种通过手术切除卵巢的操作，一直是通过动物实验了解雌激素缺乏情况的宝贵工具。尽管卵巢切除方案多种多样，但本章旨在提供一份在小鼠身上进行卵巢切除术的全面指南。此外，还将描述异氟烷麻醉系统、术后护理及手术成功评估。我们强调所有操作必须由合格且经过培训的专业人员进行，遵循伦理和安全原则。
