Laboratory of Genomic Instability and Diseases, Department of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, 751023, India.
Curr Genet. 2019 Jun;65(3):649-656. doi: 10.1007/s00294-018-0918-5. Epub 2018 Dec 8.
DNA polymerases are evolved to extend the 3'-OH of a growing primer annealed to a template DNA substrate. Since replicative DNA polymerases have a limited role while replicating structurally distorted template, translesion DNA polymerases mostly from Y-family come to the rescue of stalled replication fork and maintain genome stability. DNA polymerase eta is one such specialized enzyme whose function is directly associated with casual development of certain skin cancers and chemo-resistance. More than 20 years of extensive studies are available to support TLS activities of Polη in bypassing various DNA lesions, in addition, limited but crucial growing evidence also exist to suggest Polη possessing TLS-independent cellular functions. In this review, we have mostly focused on non-TLS activities of Polη from different organisms including our recent findings from pathogenic yeast Candida albicans.
DNA 聚合酶的进化是为了延伸与模板 DNA 底物退火的引物的 3'-OH。由于复制性 DNA 聚合酶在复制结构扭曲的模板时作用有限，因此来自 Y 家族的跨损伤 DNA 聚合酶大多会介入停滞的复制叉并维持基因组稳定性。DNA 聚合酶 eta 就是这样一种特殊的酶，其功能与某些皮肤癌和化疗耐药性的偶然发生直接相关。超过 20 年的广泛研究为 Polη 绕过各种 DNA 损伤的 TLS 活性提供了支持，此外，也有有限但至关重要的新证据表明 Polη 具有与 TLS 无关的细胞功能。在这篇综述中，我们主要关注来自不同生物体的 Polη 的非 TLS 活性，包括我们最近从致病性酵母白色念珠菌中获得的发现。
