Department of Biotechnology, College of Fisheries Science, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan, 48513, Republic of Korea.
Department of Fisheries and Marine Bioscience, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, 8100, Bangladesh.
Appl Biochem Biotechnol. 2019 Jun;188(2):498-513. doi: 10.1007/s12010-018-02930-x. Epub 2018 Dec 10.
The immunomodulatory effects of Monascus-fermented Saccharina japonica extract on anti- and pro-inflammatory cytokines gene expression of THP-1 cells were evaluated. Extracts of fermented samples showed higher phenolic, flavonoid, protein, and reducing sugar contents than unfermented one. Fermented samples were rich in many bioactive compounds determined by GC-MS analyses and showed cell viability greater than 85% in MTS assay. Regarding the anti-inflammatory and pro-inflammatory activities of the different samples, Q-PCR analyses revealed that IL-10 gene expression in THP-1 cells was significantly higher (p < 0.05) in cells treated with the SjMp or SjMk sample than those treated with the unfermented sample. Cells treated with the SjMp extract or lipopolysaccharide (LPS) showed significantly (p < 0.05) higher relative gene expression of IL-4 cytokine than cells treated with SjMk or SjU extracts. The relative gene expression of IFN-α was higher in cells treated with SjMp followed by LPS, SjMk, and SjU. TGF-β expression was higher in LPS-stimulated cells followed by SjMk and other samples. Cells treated with SjMp exhibited significantly higher pro-inflammatory (IL-6, IL-8, TNF-α, and NF-κB) cytokine gene expression than cells treated with SjU. These results revealed that extracts from S. japonica fermented with Monascus spp. regulate cytokine gene expression. Graphical abstract ᅟ.
评估了红曲霉发酵的昆布提取物对 THP-1 细胞抗炎和促炎细胞因子基因表达的免疫调节作用。发酵样品的酚类、黄酮类、蛋白质和还原糖含量均高于未发酵样品。发酵样品富含多种通过 GC-MS 分析确定的生物活性化合物，MTS 测定显示细胞活力大于 85%。关于不同样品的抗炎和促炎活性，qPCR 分析表明，与未发酵样品相比，用 SjMp 或 SjMk 样品处理的 THP-1 细胞中 IL-10 基因的表达显著更高（p<0.05）。用 SjMp 提取物或脂多糖（LPS）处理的细胞中，IL-4 细胞因子的相对基因表达显著高于用 SjMk 或 SjU 提取物处理的细胞（p<0.05）。用 SjMp 处理的细胞中 IFN-α的相对基因表达高于 LPS、SjMk 和 SjU 处理的细胞。LPS 刺激的细胞中 TGF-β的表达高于 SjMk 和其他样品。用 SjMp 处理的细胞表现出明显更高的促炎（IL-6、IL-8、TNF-α 和 NF-κB）细胞因子基因表达，高于用 SjU 处理的细胞。这些结果表明，昆布与红曲霉发酵的提取物可调节细胞因子基因表达。
