Department of Pharmacology, Ningxia Medical University, Yinchuan, 750004, China.
Key Laboratory of Hui Ethnic Medicine Modernization, The Ministry of Education, Ningxia Medical University, Yinchuan, 750004, China.
Biomed Pharmacother. 2019 Feb;110:561-570. doi: 10.1016/j.biopha.2018.12.012. Epub 2018 Dec 8.
Spermatogenic dysfunction is a common complication in men with diabetes and is the most important manifestation of diabetes-related male reproduction damage. Astragalin (AG) is one of the main flavonoids from Cuscuta chinensis, which has rich pharmacological activities. This study aimed to establish whether AG may contribute to the recovery from spermatogenic dysfunction. AG (3.3, 10 and 30 mg/kg) and Clomiphene (5 mg/kg) were orally administered to streptozotocin-induced diabetic male mice for 8 weeks. After the experiments performed, reproductive organs, sperm parameters and histomorphological changes were analysed. Antioxidant and anti-inflammatory capacity were estimated in testicular tissues. The results revealed that AG significantly improved the reproductive organs, sperm parameters and testicular morphology to different degrees in diabetic mice. Nitric oxide (NO) and malondialdehyde (MDA) levels were significantly reduced, and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), markedly increased in the testicular tissue after AG was administered. Interestingly, AG also downregulated the protein expressions of tumour necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) in testes. In conclusion, AG is a potential beneficial agent to protect diabetic-induced spermatogenic dysfunction in male mice by increasing antioxidant enzymes activities and inhibiting inflammation.
生精功能障碍是糖尿病男性的常见并发症，也是糖尿病相关男性生殖损害的最重要表现。毛蕊异黄酮是菟丝子中的主要黄酮类化合物之一，具有丰富的药理活性。本研究旨在探讨毛蕊异黄酮是否有助于恢复生精功能障碍。将毛蕊异黄酮（3.3、10 和 30mg/kg）和氯米酚（5mg/kg）灌胃给予链脲佐菌素诱导的糖尿病雄性小鼠 8 周。实验结束后，分析生殖器官、精子参数和组织形态学变化。检测睾丸组织的抗氧化和抗炎能力。结果表明，毛蕊异黄酮可不同程度地改善糖尿病小鼠的生殖器官、精子参数和睾丸形态。NO 和 MDA 水平显著降低，睾丸组织中 SOD、GSH-Px 和 CAT 的活性显著增加。有趣的是，毛蕊异黄酮还下调了睾丸中肿瘤坏死因子-α（TNF-α）和诱导型一氧化氮合酶（iNOS）的蛋白表达。综上所述，毛蕊异黄酮通过增加抗氧化酶活性和抑制炎症，可能成为一种有潜力的保护糖尿病诱导的雄性小鼠生精功能障碍的药物。
