Department of Dental Clinics, Oral Pathology, and Oral Surgery, School of Dentistry, Federal University of Minas Gerais, Antônio Carlos Avenue, 6627, Pampulha, PO Box 359, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
Department of Dentistry, Periodontics Research Division, University of Taubaté, Taubaté, São Paulo, Brazil.
Clin Oral Investig. 2019 Sep;23(9):3517-3526. doi: 10.1007/s00784-018-2770-x. Epub 2018 Dec 11.
To longitudinally evaluate the effects of compliance during periodontal maintenance therapy (PMT) on cytokines levels and its relation to periodontal status.
Ninety-one eligible individuals were selected from a 6-year prospective study with 212 individuals in PMT. From this total, 28 regular compliers (RC) were randomly selected and matched for age and gender with 28 irregular compliers (IC). All participants were non-smokers and non-diabetic. Periodontal parameters and gingival crevicular fluid samples were collected in 5 times: T1 [prior to active periodontal therapy (APT)], T2 (after APT), T3 (2 years), T4 (4 years), and T5 (6 years). Levels of IL-6, IL-10, IL-1β, TNF-α, and MMP-8 were quantified through ELISA.
RC presented better clinical periodontal status over time when compared to IC. A significant reduction in the levels of IL-1β, TNF-α, MMP-8, and IL-6 was observed among RC along time (from T1 to T5). Levels of IL-1 were similar among groups. By contrast, levels of IL-6 and TNF-α increased over time in IC individuals. Levels of IL-10 increased among RC and reduced among IC.
The inflammatory cytokines IL-1, TNF-α, IL-6, and MMP-8 were correlated with worse clinical parameters among IC, while IL-10 was associated with an improvement in clinical parameters among RC. These results reinforce the role of these cytokines in the pathogenesis of periodontitis, as well as their role as markers to monitoring the progression of the periodontitis.
Regular compliance during 6-year period the PMT sustained clinical and immunological benefits obtained after active periodontal therapy.
纵向评估牙周维护治疗（PMT）期间依从性对细胞因子水平的影响及其与牙周状况的关系。
从一项为期 6 年的前瞻性研究中选择了 91 名符合条件的个体，该研究中有 212 名个体接受 PMT。从这一总数中，随机选择了 28 名常规依从者（RC），并按年龄和性别与 28 名不规律依从者（IC）匹配。所有参与者均为非吸烟者且非糖尿病患者。在 5 个时间点收集牙周参数和龈沟液样本：T1[在主动牙周治疗（APT）之前]、T2（APT 后）、T3（2 年）、T4（4 年）和 T5（6 年）。通过 ELISA 定量测定 IL-6、IL-10、IL-1β、TNF-α 和 MMP-8 的水平。
与 IC 相比，RC 在整个时间内表现出更好的临床牙周状况。RC 随着时间的推移（从 T1 到 T5），IL-1β、TNF-α、MMP-8 和 IL-6 的水平显著降低。IL-1 的水平在各组之间相似。相比之下，IC 个体的 IL-6 和 TNF-α水平随着时间的推移而增加。IL-10 的水平在 RC 中增加，在 IC 中减少。
在 IC 中，炎症细胞因子 IL-1、TNF-α、IL-6 和 MMP-8 与较差的临床参数相关，而 IL-10 与 RC 中临床参数的改善相关。这些结果强化了这些细胞因子在牙周炎发病机制中的作用，以及它们作为监测牙周炎进展的标志物的作用。
在 6 年的 PMT 期间，定期依从性维持了主动牙周治疗后获得的临床和免疫学益处。
