Escalona Laura A, Mastromatteo-Alberga Patrizzia, Correnti María
Invest Clin. 2016 Jun;57(2):131-142.
The purpose of the present research was to determine the levels of IL- 1a, IL- 1 P, TNF-a, IL-6, IL-6sR, IL-8, IL-10, MMP-3 and EMP-8 in gingival crevicular fluid (GCF) of subjects with chronic periodontitis. Clinical measurements were carried out in 20 patients with chronic periodontitis and 11 periodontally healthy controls. The clinical indexes evalua- ted were: gingival index (GI), plaque index (PI), bleeding on probing (BOP), probing depth (PD) and clinical attachment loss (CAL); the measurements were taken at six sites per tooth in all teeth in each subject. GCF samples were taken from one tooth per quadrant, and the levels of mediators were measured using an ELISA test. Statistically significant differences were observed between patients and control group in relation to all clinical parameters evaluated (p<0.05). The gingival concentrations, in pg/mL, of IL-la (patients: 239.06 ± 65.5 vs control: 97.79 ± 15.81), IL-10 (patients: 157.19 ± 36.4 vs control: 63.44 ± 19.04), TNF-a (patients: 10.87 ± 1.7 vs control: 1.15 ± 0.84), IL-6 (patients: 3.77 ± 1.7 vs control: 0.43 ± 0.22), IL-6Sr (patients: 655.59 ± 185.8 vs control: 73.59 ± 23.18), IL-8 (patients: 496.3 ± 155.3 vs control: 206.13 ± 46.63), IL-10 (patients: 10.75 ± 3.6 vs control: 2.41 ± 0.57), MMP-3 (patients: 3531 ± 1558.2 vs control: 724.84 ± 289.51) and MMiP-8 (patients: 8231.70 ± 1279.2 vs control: 1534.67± 814.90) were significantly greater in patients with periodontal disease than in the control group (p<0.00 1). The higher levels of the cytokines and metalloproteinases obtained in this study were significantly associated with the severity of the periodontal disease.
本研究的目的是测定慢性牙周炎患者龈沟液（GCF）中白细胞介素-1α（IL-1α）、白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、白细胞介素-6可溶性受体（IL-6sR）、白细胞介素-8（IL-8）、白细胞介素-10（IL-10）、基质金属蛋白酶-3（MMP-3）和上皮膜蛋白-8（EMP-8）的水平。对20例慢性牙周炎患者和11例牙周健康对照者进行了临床测量。评估的临床指标包括：牙龈指数（GI）、菌斑指数（PI）、探诊出血（BOP）、探诊深度（PD）和临床附着丧失（CAL）；在每个受试者的所有牙齿上，每颗牙齿的六个部位进行测量。从每个象限的一颗牙齿采集GCF样本，并使用酶联免疫吸附测定（ELISA）试验测量介质水平。在患者和对照组之间，在所评估的所有临床参数方面观察到统计学上的显著差异（p<0.05）。牙周病患者龈沟液中IL-1α（患者：239.06±65.5 vs对照：97.79±15.81）、IL-10（患者：157.19±36.4 vs对照：63.44±19.04）、TNF-α（患者：10.87±1.7 vs对照：1.15±0.84）、IL-6（患者：3.77±1.7 vs对照：0.43±0.22）、IL-6Sr（患者：655.59±185.8 vs对照：73.59±23.18）、IL-8（患者：496.3±155.3 vs对照：206.13±46.63）、IL-10（患者：10.75±3.6 vs对照：2.41±0.57）、MMP-3（患者：3531±1558.2 vs对照：724.84±289.51）和MMiP-8（患者：8231.70±1279.2 vs对照：1534.67±814.90）的浓度（单位：pg/mL）显著高于对照组（p<0.001）。本研究中获得的细胞因子和金属蛋白酶的较高水平与牙周病的严重程度显著相关。
