Pereira H G, Flewett T H, Candeias J A, Barth O M
Department of Virology, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
J Gen Virol. 1988 Nov;69 ( Pt 11):2749-54. doi: 10.1099/0022-1317-69-11-2749.
Examination of the intestinal contents of free-living Oryzomys nigripes rats by PAGE revealed two sharply defined bands that could be stained by ethidium bromide or by silver nitrate with comparable intensities. The molecules forming these bands were susceptible to digestion by pancreatic RNase A but not by RNase T1 or by DNase I. Their lengths were estimated to be about 2.6 and 1.5 kbp, respectively, by comparison with rotavirus SA11 genome segments. They cosedimented in CsCl gradients at a density of 1.39 to 1.40 g/ml, together with uniform particles approximately 35 nm in diameter with indistinct surface structure. It is suggested that these particles represent an as yet undescribed virus with a bisegmented double-stranded RNA genome, for which the name 'picobirnavirus' is proposed.
通过聚丙烯酰胺凝胶电泳（PAGE）对自由生活的黑稻鼠肠道内容物进行检测，发现有两条清晰界定的条带，可用溴化乙锭或硝酸银染色，染色强度相当。形成这些条带的分子易被胰核糖核酸酶A消化，但不被核糖核酸酶T1或脱氧核糖核酸酶I消化。与轮状病毒SA11基因组片段相比，它们的长度估计分别约为2.6和1.5千碱基对。它们在氯化铯梯度中以1.39至1.40克/毫升的密度与直径约35纳米、表面结构不清晰的均匀颗粒一起沉降。有人提出，这些颗粒代表一种尚未描述的病毒，其基因组为双节段双链RNA，为此建议将其命名为“微小双RNA病毒”。
