Ludert J E, Hidalgo M, Gil F, Liprandi F
Laboratorio Biologia de Virus, Instituto Venezolano de Investigaciones Cientificas (IVIC), Caracas, Venezuela.
Arch Virol. 1991;117(1-2):97-107. doi: 10.1007/BF01310495.
Polyacrylamide gel electrophoresis of nucleic acids extracted from porcine faecal samples revealed in several samples the presence of two discrete bands. The bands were resistant to digestion with of DNase I and RNase T1, but not with RNase A in low salt conditions, indicating that they consisted of double stranded (ds) RNA. The two bands from different samples varied in sizes, in a range between 2.4-2.6 kbp and 1.7-1.9 kbp for the slow and fast moving band respectively. The bands cosedimented in CsCl gradients at an average density of 1.415 g/ml with icosahedral virus particles of a diameter of 34 nm and a triangulation number equal to 3. Aggregates of virus, which appeared to be immunocomplexes, were seen in one sample. From 244 faecal samples collected in one farm, 27 (11.1%) were found to contain the characteristic dsRNA pattern, with a higher prevalence in samples from animals 15 to 35 days old. The agent was equally distributed among samples from diarrhoeic or non-diarrhoeic animals. These results confirm the circulation among pigs of a novel virus, possibly of vertebrates, with a bisegmented double stranded RNA genome, similar to viruses previously described in humans, wild rats, guinea pigs, pigs, and chickens, for which the name "picobirnavirus" has been proposed.
对从猪粪便样本中提取的核酸进行聚丙烯酰胺凝胶电泳分析，结果显示在多个样本中存在两条清晰的条带。这些条带对脱氧核糖核酸酶I（DNase I）和核糖核酸酶T1（RNase T1）具有抗性，但在低盐条件下对核糖核酸酶A（RNase A）敏感，这表明它们由双链（ds）RNA组成。来自不同样本的两条条带大小各异，慢速移动条带的大小在2.4 - 2.6千碱基对（kbp）之间，快速移动条带的大小在1.7 - 1.9 kbp之间。这些条带在氯化铯（CsCl）梯度中与直径为34纳米、三角剖分数等于3的二十面体病毒颗粒以平均密度1.415克/毫升共同沉降。在一个样本中发现了病毒聚集体，似乎是免疫复合物。在一个猪场收集的244份粪便样本中，有27份（11.1%）被发现含有特征性的双链RNA模式，在15至35日龄动物的样本中患病率更高。该病原体在腹泻或非腹泻动物的样本中分布均匀。这些结果证实了一种新型病毒在猪群中的传播，这种病毒可能是脊椎动物病毒，具有双节段双链RNA基因组，类似于先前在人类、野生大鼠、豚鼠、猪和鸡中描述的病毒，为此已提议将其命名为“微小双RNA病毒”。
