College of Life Science, Capital Normal University, 100048, Beijing, China.
J Plant Physiol. 2019 Jan;232:270-283. doi: 10.1016/j.jplph.2018.11.011. Epub 2018 Nov 16.
Integrated proteome analyses revealed differentially accumulated proteins in the non-leaf green organs in wheat glume and awn that play important roles in photosynthesis and drought resistance. Two non-leaf green organs in wheat, glume and awn, have photosynthetic potential, contribute to grain yield, and also play roles in resistance to adverse conditions. We performed the first integrated proteome analysis of wheat glume and awn in response to water deficit. Water deficit caused a significant decrease in important agronomic traits and grain yield. A total of 120 and 77 differentially accumulated protein (DAP) spots, representing 100 and 67 unique proteins responsive to water deficit, were identified by two-dimensional difference gel electrophoresis (2D-DIGE) in glumes and awns, respectively, of the elite Chinese bread wheat cultivar Zhongmai 175. The DAPs of both organs showed similar functional classification and proportion and were mainly involved in photosynthesis, detoxification/defense, carbon/energy metabolism, and proteometabolism. Comparative proteome analyses revealed many more drought-responsive DAP spots in glumes than in awns, which indicate that glumes underwent more proteome changes in response to water deficit. The main DAPs involved in photosynthesis and carbon metabolism were significantly downregulated, whereas those related to detoxification/defense and energy metabolism were markedly upregulated under water deficit. The potential functions of the identified DAPs revealed an intricate interaction network that responds synergistically to drought stress during grain development. Our results from the proteome perspective illustrate the potential roles of wheat non-leaf green organs glume and awn in photosynthetic and defensive responses under drought stress.
整合的蛋白质组分析揭示了小麦颖壳和芒中差异积累的非绿叶绿色器官蛋白，这些蛋白在光合作用和抗旱性中起着重要作用。小麦的两个非绿叶绿色器官，颖壳和芒，具有光合作用的潜力，有助于籽粒产量，并且在抵抗不利条件方面也起着作用。我们首次对小麦颖壳和芒在水分亏缺下的蛋白质组进行了综合分析。水分亏缺导致重要农艺性状和籽粒产量显著下降。通过二维差异凝胶电泳（2D-DIGE）分别在优质中国面包小麦品种中麦 175 的颖壳和芒中鉴定出 120 和 77 个差异积累蛋白（DAP）点，分别代表 100 和 67 个对水分亏缺有响应的独特蛋白。两个器官的 DAPs 表现出相似的功能分类和比例，主要涉及光合作用、解毒/防御、碳/能量代谢和蛋白质代谢。比较蛋白质组分析表明，颖壳中比芒中具有更多的抗旱响应 DAP 点，这表明颖壳在响应水分亏缺时经历了更多的蛋白质组变化。参与光合作用和碳代谢的主要 DAP 显著下调，而与解毒/防御和能量代谢相关的 DAP 显著上调。鉴定出的 DAPs 的潜在功能揭示了一个错综复杂的相互作用网络，该网络在籽粒发育过程中协同响应干旱胁迫。我们从蛋白质组的角度得出的结果说明了小麦非绿叶绿色器官颖壳和芒在干旱胁迫下光合作用和防御反应中的潜在作用。
