Yu Yanbao, Tsitrin Tamara, Singh Harinder, Doerfert Sebastian N, Sizova Maria V, Epstein Slava S, Pieper Rembert
J. Craig Venter Institute, 9605 Medical Center Drive, Rockville, MD 20850, USA.
College of Science, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
Proteomes. 2018 Dec 9;6(4):52. doi: 10.3390/proteomes6040052.
, a Gram-positive anaerobic coccoid rod colonizing the human urinary tract, belongs to the taxonomic class of Actinobacteria. We identified as a cohabitant of urethral catheter biofilms (CB). The CBs also harbored more common uropathogens, such as and , supporting the notion that is adapted to a life style in polymicrobial biofilms. We isolated a clinical strain from a blood agar colony and used 16S rRNA gene sequencing and shotgun proteomics to confirm its identity as . We characterized this species by quantitatively comparing the bacterial proteome derived from in vitro growth with that of four clinical samples. The functional relevance of proteins with emphasis on nutrient import and the response to hostile host conditions, showing evidence of neutrophil infiltration, was analyzed. Two putative subtilisin-like proteases and a heme/oligopeptide transporter were abundant in vivo and are likely important for survival and fitness in the biofilm. Proteins facilitating uptake of xylose/glucuronate and oligopeptides, also highly expressed in vivo, may feed metabolites into mixed acid fermentation and peptidolysis pathways, respectively, to generate energy. A polyketide synthase predicted to generate a secondary metabolite that interacts with either the human host or co-colonizing microbes was also identified. The product of the PKS enzyme may contribute to fitness and persistence in the CBs.
[细菌名称]是一种定殖于人类尿道的革兰氏阳性厌氧球状杆菌，属于放线菌纲。我们鉴定出[细菌名称]是尿道导管生物膜（CB）的共生菌。这些CB中还含有更常见的尿路病原体，如[其他细菌名称1]和[其他细菌名称2]，这支持了[细菌名称]适应多微生物生物膜生活方式的观点。我们从血琼脂菌落中分离出一株临床菌株，并使用16S rRNA基因测序和鸟枪法蛋白质组学来确认其为[细菌名称]。我们通过定量比较体外生长的细菌蛋白质组与四个临床样本的蛋白质组来表征该物种。分析了重点在于营养物质摄取和对宿主不利条件反应的蛋白质的功能相关性，显示出中性粒细胞浸润的证据。两种假定的枯草杆菌蛋白酶样蛋白酶和一种血红素/寡肽转运蛋白在体内含量丰富，可能对生物膜中的生存和适应性很重要。促进木糖/葡萄糖醛酸和寡肽摄取的蛋白质在体内也高度表达，可能分别将代谢产物输入混合酸发酵和肽分解途径以产生能量。还鉴定出一种预测可产生与人类宿主或共定殖微生物相互作用的次生代谢产物的聚酮合酶。聚酮合酶的产物可能有助于[细菌名称]在CB中的适应性和持久性。
