Ling I T, Wilson R J
National Institute for Medical Research, London, U.K.
Mol Biochem Parasitol. 1988 Oct;31(1):47-56. doi: 10.1016/0166-6851(88)90144-2.
Schizonts of Plasmodium falciparum, grown either in normal or glucose-6-phosphate dehydrogenase (G6PDH) deficient human red cells, contain an electrophoretically slow-moving form of G6PDH. The slow mobility of the G6PDH in non-dissociating polyacrylamide gels is due to its large size (Mr ca. 450,000) rather than to its charge. The activity of this enzyme was less than 10% of normal red cell G6PDH. These characteristics of the parasite-associated G6PDH were unaltered when parasites were grown in red cells from a G6PDH A+B+ heterozygote or following the introduction of a heterologous G6PDH into resealed ghosts. Differential absorption of the parasite-associated and red cell G6PDHs was demonstrated with antisera containing antibodies to red cell G6PDH. These studies show that a novel form of G6PDH is associated with P. falciparum in normal red cells without the requirements for induction by one or several cycles of multiplication in G6PDH deficient red cells.
恶性疟原虫的裂殖体，无论是在正常的还是葡萄糖-6-磷酸脱氢酶（G6PDH）缺乏的人红细胞中生长，都含有一种电泳迁移缓慢的G6PDH形式。在非解离聚丙烯酰胺凝胶中，G6PDH迁移缓慢是由于其分子量大（约450,000道尔顿），而非电荷原因。这种酶的活性不到正常红细胞G6PDH的10%。当寄生虫在G6PDH A+B+杂合子的红细胞中生长或在重新封闭的血影中引入异源G6PDH后，寄生虫相关G6PDH的这些特性并未改变。用含有抗红细胞G6PDH抗体的抗血清证明了寄生虫相关G6PDH和红细胞G6PDH的差异吸收。这些研究表明，一种新型的G6PDH与正常红细胞中的恶性疟原虫相关，无需在G6PDH缺乏的红细胞中经过一个或几个增殖周期诱导。
