Laboratorio de Fitofarmacología, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calzada México-Xochimilco 101, Col. San Lorenzo Huipulco, Tlalpan, 14370 Ciudad de México, Mexico.
Instituto Politécnico Nacional CNMN, Luis Enrique Erro s/n, Unidad Prof. Adolfo López Mateos, Gustavo A. Madero, 07738 Ciudad de México, Mexico.
J Ethnopharmacol. 2019 Mar 1;231:453-463. doi: 10.1016/j.jep.2018.10.030. Epub 2018 Oct 26.
Piper auritum Kunth is employed as an aphrodisiac in the traditional medicine, but corroborative evidence for such effect is scarce.
The pro-sexual effect of an aqueous extract of P. auritum and its possible mechanisms were analyzed in two paradigms of male sexual function.
Effects of an aqueous extract of P. auritum (PA, single administration) were investigated in the fictive ejaculation, and copulatory behavior paradigms in sexually sluggish male rats. WAY 100635 (antagonist of 5-HT receptors), atosiban (antagonist of oxytocinergic receptors), L-NAME (inhibitor of the nitric oxide synthase) and baclofen (antagonist of GABA receptors) were used as pre-treatments in order to investigate the role of different neurotransmitter systems in PA actions. Chemical profile of PA was determined by Gases Chromatography and Ultra Performance Chromatography-Electrospray Ionization-Masses Spectrometry (UPLC-ESI-MS).
In males with retarded ejaculation, PA stimulated ejaculatory behavior and recovered electromyographic activity of pelvic musculature participating in seminal emission and ejaculation. All pre-treatments blocked stimulating effects of PA on the fictive ejaculation; additionally WAY 100635 interfered with PA actions on ejaculatory behavior. Safrol, apigenin dimethylether, myristicin, vaccihein A, sakuranin and sakuranetin flavonoids, were main constituents of PA, with possible participation in its pro-sexual effects.
Pro-sexual effects of P. auritum elicited at level of ejaculation were mediated by several neurotransmitter systems, among which serotonin and its 5-HT receptors play an important role. Present findings support P. auritum reputation as an aphrodisiac, with potential use in delayed ejaculation disorder.
Piper auritum Kunth 作为传统医学中的壮阳药使用，但缺乏对此功效的确证证据。
分析 Piper auritum 水提物对雄性性功能的两个模型的促性作用及其可能的机制。
在性机能减退雄性大鼠的拟射精和交配行为模型中，研究了 Piper auritum（PA，单次给药）水提物的促性作用。WAY 100635（5-HT 受体拮抗剂）、阿托西班（催产素受体拮抗剂）、L-NAME（一氧化氮合酶抑制剂）和巴氯芬（GABA 受体拮抗剂）被用作预处理，以研究不同神经递质系统在 PA 作用中的作用。PA 的化学特征通过气相色谱和超高效液相色谱-电喷雾离子化-质谱联用（UPLC-ESI-MS）确定。
在射精延迟的雄性中，PA 刺激了射精行为，并恢复了参与精液排放和射精的骨盆肌肉的肌电图活动。所有预处理均阻断了 PA 对拟射精的刺激作用；此外，WAY 100635 还干扰了 PA 对射精行为的作用。水芹烯、芹菜素二甲醚、肉豆蔻醚、伏康酸 A、樱黄素和樱黄素类黄酮是 PA 的主要成分，可能参与其促性作用。
PA 在射精水平上产生的促性作用是由几个神经递质系统介导的，其中 5-羟色胺及其 5-HT 受体起着重要作用。目前的研究结果支持了 Piper auritum 作为壮阳药的声誉，具有治疗延迟射精障碍的潜力。
