Guo Honghong, Xue Shuhong, Nasir Mubasher, Lv Jialong, Gu Jei
Key Laboratory of Plant Nutrition and Agri-Environment in Northwest China, College of Natural Resources and Environment, Northwest A&F University, Yangling, China.
State Key Laboratory Base of Eco-Hydraulic Engineering in Arid Area, Xi'an University of Technology, Xi'an, China.
Front Microbiol. 2018 Nov 28;9:2722. doi: 10.3389/fmicb.2018.02722. eCollection 2018.
The effects of bentonite (BT), a commonly used heavy metal deactivator, on the ARGs and microbial communities in soils and lettuce systems contaminated by heavy metals and antibiotics are unclear. A study was conducted to investigate the effect of BT on the mobility of antibiotic resistance genes in oxytetracycline and cadmium contaminated soil. Results showed that the addition of BT reduced the accumulation of OTC and ARGs in the soil and lettuce roots, but increased the abundance of ARGs in lettuce leaves, and increase the risk of human pathogenic bacteria (HPB) transferring to lettuce leaves. Redundancy analysis showed that environmental factors (OTC, HO, SOM, and pH) were the dominant factors that influence the distribution of ARGs and . Network analysis showed that Proteobacteria and Bacteroidetes were the major host bacteria which caused changes in ARGs and . There were significant positive correlations between and , and a large number of HPB. The co-occurrence of with some ARGs (, and ), may threaten human health due to the dispersion of ARGs via horizontal gene transfer.
膨润土（BT）是一种常用的重金属钝化剂，其对重金属和抗生素污染的土壤及生菜系统中的抗生素抗性基因（ARGs）和微生物群落的影响尚不清楚。开展了一项研究以调查膨润土对土霉素和镉污染土壤中抗生素抗性基因迁移的影响。结果表明，添加膨润土减少了土壤和生菜根部中土霉素和ARGs的积累，但增加了生菜叶片中ARGs的丰度，并增加了人类病原菌（HPB）转移到生菜叶片的风险。冗余分析表明，环境因素（土霉素、过氧化氢、土壤有机质和pH值）是影响ARGs分布的主要因素。网络分析表明，变形菌门和拟杆菌门是导致ARGs变化的主要宿主细菌。[此处原文似乎有缺失内容]与[此处原文似乎有缺失内容]和大量HPB之间存在显著正相关。[此处原文似乎有缺失内容]与一些ARGs（[此处原文似乎有缺失内容]、[此处原文似乎有缺失内容]和[此处原文似乎有缺失内容]）的共存，可能由于ARGs通过水平基因转移扩散而威胁人类健康。
