Department of Chemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.
Research and Development, Beiersdorf AG, Hamburg, Germany.
Pigment Cell Melanoma Res. 2019 May;32(3):441-447. doi: 10.1111/pcmr.12754. Epub 2018 Dec 11.
Exposure to excess ultraviolet (UV) A radiation induces the degradation/modification of both eumelanin and pheomelanin that may be deleterious to pigmented tissues. Although the spectral distribution of solar energy comprises nearly half of visible light (VL), few studies have been conducted to examine the role of VL in the photodegradation of both types of melanin, either VL alone or in combination with UVA. In this study, we examined the effects of physiological doses of VL (150 to 300 J cm ) alone or in combination with a physiological dose of UVA (20 J cm ) in normal human epidermal melanocytes. The degradation/modification of melanin structures was evaluated by our chemical degradation-high performance liquid chromatography methods. The results show that VL accelerates UVA-induced changes in the structural features of both eumelanin and pheomelanin, although VL or UVA alone induced only minor changes in melanin structure. The differential spectral method provides support for the additive effects of VL.
过量的紫外线（UV）A 辐射会导致真黑素和褐黑素的降解/修饰，这可能对色素组织有害。尽管太阳能的光谱分布包含近一半的可见光（VL），但很少有研究探讨 VL 在两种黑素的光降解中的作用，无论是 VL 单独作用还是与 UVA 联合作用。在这项研究中，我们检查了生理剂量的 VL（150 至 300 J cm ）单独或与生理剂量的 UVA（20 J cm ）联合作用对正常人表皮黑素细胞的影响。通过我们的化学降解-高效液相色谱方法评估黑素结构的降解/修饰。结果表明，VL 加速 UVA 诱导的真黑素和褐黑素结构特征的变化，尽管 VL 或 UVA 单独作用仅引起黑素结构的微小变化。差谱法为 VL 的相加效应提供了支持。
