Drobac Milica, Petrović Silvana, Milenković Marina, Couladis Maria, Kukić-Marković Jelena, Niketić Marjan
Nat Prod Commun. 2017 Mar;12(3):445-448.
The compositions of hydrodistillated essential oils of Laser trilobum (L.) Borkh. rhizomes and fruits from Serbia, were investigated using GC and GC/MS. In the dark-blue rhizome oil forty-six compounds (93.1% of the total oil) were identified, with a-pinene (31.5%), y-terpinene (9.0%), p-cymene (7.9%), β-pinene (6.1%) and 1,4-dimethylazalene (6.0%) as the major components. In the colorless fruits oil, twenty components (96.8% of the total oil) were identified, and the main constituents were limonene (51.6%) and perillaldehyde (26.8%). The antimidrobial activity of the oils was tested using the broth microdilution method against nine bacterial and two fungal strains. The oils revealed significant antimicrobial effect, mainly better than that of thymol, used as a reference compound. The strongest activity was recorded for the rhizome oil against Escherichia coli, Klebsiella pneumoniae and Candida albicans (MICs=25 μg/mL), and the fruit oil against C. albicans ATCC 10259 (MIC=12.5 μg/mL).
采用气相色谱（GC）和气相色谱 - 质谱联用（GC/MS）技术，对采自塞尔维亚的三裂叶滨藜（Laser trilobum (L.) Borkh.）根茎和果实的水蒸馏精油成分进行了研究。在深蓝色的根茎精油中，鉴定出了46种化合物（占精油总量的93.1%），主要成分包括α-蒎烯（31.5%）、γ-萜品烯（9.0%）、对伞花烃（7.9%）、β-蒎烯（6.1%）和1,4 - 二甲基氮杂萘（6.0%）。在无色的果实精油中，鉴定出了20种成分（占精油总量的96.8%），主要成分是柠檬烯（51.6%）和紫苏醛（26.8%）。采用肉汤微量稀释法，对这两种精油针对9种细菌菌株和2种真菌菌株的抗菌活性进行了测试。结果表明，这两种精油具有显著的抗菌效果，主要优于用作参考化合物的百里酚。根茎精油对大肠杆菌、肺炎克雷伯菌和白色念珠菌的抗菌活性最强（最低抑菌浓度MICs = 25μg/mL），果实精油对白色念珠菌ATCC 10259的抗菌活性最强（最低抑菌浓度MIC = 12.5μg/mL）。
