Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, China.
Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, China.
Biomed Pharmacother. 2019 Jan;109:2434-2440. doi: 10.1016/j.biopha.2018.11.132. Epub 2018 Nov 30.
Acute respiratory distress syndrome is associated with a mortality of 45%. The authors investigated the possible mechanisms and effect of vascular endothelial growth factor on alveolar epithelial barrier permeability in acute respiratory distress syndrome mice model.
Eighty Male BALB/c mice were randomly assigned to four group: PBS group, LPS group, sFlt group, or LPS + sFlt group. The levels of vascular endothelial growth factor and total protein in bronchoalveolar lavage fluid were compared, together with lung injury score and the histopathology of alveolar epithelial barrier. The expressions of vascular endothelial growth factor and tight junction proteins mRNA in lung tissue were also studied.
Lipopolysaccharide (LPS) inhaling was accompanied with increasing lung vascular endothelial growth factor (VEGF) expression. Anti-VEGF with soluble fms-like tyrosine kinase-1 (sFlt-1) attenuated the lung injury effectively.
Our data indicate that anti-vascular endothelial growth factor with soluble fms-like tyrosine kinase-1 could maintain the normal structure and function of respiratory membrane in acute respiratory distress syndrome mice model and might be a suitable therapeutic tool for the treatment of acute respiratory distress syndrome.
急性呼吸窘迫综合征的死亡率为 45%。作者研究了血管内皮生长因子（VEGF）对急性呼吸窘迫综合征小鼠模型肺泡上皮屏障通透性的可能机制和影响。
80 只雄性 BALB/c 小鼠随机分为四组：PBS 组、LPS 组、sFlt 组或 LPS+sFlt 组。比较各组支气管肺泡灌洗液中血管内皮生长因子和总蛋白水平，肺损伤评分和肺泡上皮屏障的组织病理学。还研究了肺组织中血管内皮生长因子和紧密连接蛋白 mRNA 的表达。
LPS 吸入后肺血管内皮生长因子（VEGF）表达增加。用可溶性 Fms 样酪氨酸激酶-1（sFlt-1）拮抗 VEGF 可有效减轻肺损伤。
我们的数据表明，用可溶性 Fms 样酪氨酸激酶-1 拮抗血管内皮生长因子可维持急性呼吸窘迫综合征小鼠模型呼吸膜的正常结构和功能，可能是治疗急性呼吸窘迫综合征的一种合适的治疗工具。
