Department of Pediatrics, Hangzhou First People's Hospital, 261 Huansha Road, Hangzhou, 310006, China.
Lung. 2015 Dec;193(6):1029-36. doi: 10.1007/s00408-015-9803-x. Epub 2015 Sep 28.
To test the hypothesis that exogenous administration of vascular endothelial growth factor (VEGF) promotes lung repair in acute lung injury (ALI).
ALI was induced by intranasal lipopolysaccharide (LPS) administration in mice, followed by different treatment protocols for 7 days in 3 groups (n = 6, each) including the LPS, the VEGF and the anti-VEGF group. At day 7, peripheral blood and lungs were collected. Lung wet-to-dry (W/D) ratio and lung injury score were measured. Immunohistochemistry assay was employed to detect the number of pulmonary vessels. Circulating endothelial progenitor cells (EPCs) was detected using flow cytometric analysis, and the apoptosis of lung cells was determined by TUNEL staining.
VEGF treatment reduced W/D ratio and pulmonary neutrophil infiltration in the VEGF group compared with the LPS group. The treatment of VEGF increased the number of pulmonary vessels, and significantly increased the number of circulating EPC cells. Moreover, administration of VEGF decreased the percentage of apoptotic cells in the VEGF group.
Our results suggest that VEGF may contribute to vascular endothelial repair and function as a protective factor against ALI.
验证外源性血管内皮生长因子（VEGF）是否能促进急性肺损伤（ALI）中的肺修复。
通过鼻腔内给予脂多糖（LPS）的方法在小鼠中诱导 ALI，随后在 3 组（每组 n = 6）中分别采用不同的治疗方案处理 7 天，这 3 组分别是 LPS 组、VEGF 组和抗 VEGF 组。第 7 天，收集外周血和肺脏。测量肺湿重/干重（W/D）比值和肺损伤评分。通过免疫组化检测肺血管数量。通过流式细胞术分析检测循环内皮祖细胞（EPC），通过 TUNEL 染色检测肺细胞的凋亡。
与 LPS 组相比，VEGF 治疗降低了 VEGF 组的 W/D 比值和肺中性粒细胞浸润。VEGF 的治疗增加了肺血管的数量，并显著增加了循环 EPC 细胞的数量。此外，VEGF 组的细胞凋亡比例降低。
我们的结果表明，VEGF 可能有助于血管内皮修复，并作为对抗 ALI 的保护因子。
