Discipline of Biochemistry, School of Life Sciences, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban 4000, South Africa.
Discipline of Biological Sciences, School of Life Sciences, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban 4000, South Africa.
Biomed Pharmacother. 2019 Jan;109:2441-2446. doi: 10.1016/j.biopha.2018.11.137. Epub 2018 Dec 1.
Diabetes mellitus is characterized by hyperglycaemia that results from defects in insulin secretion or insulin action and is accompanied by general disturbances metabolism. Psidium guajava (PG) leaf is known to have antidiabetic effects that include lowering of blood glucose. The aim of the study was to investigate the effect of PG leaf extract on tissue activity of glycogen synthase (GS) and glycogen phosphorylase (GP); tissue activity of hormone sensitive lipase (HSL); serum lipid profile; and serum enzyme biomarkers of tissue damage. Diabetes was induced in male Sprague-Dawley rats with a single dose of 40 mg/kg body weight streptozotocin. The aqueous extract of PG leaves was used to treat both normal and diabetic animals (400 mg/kg body weight) for 2 weeks while control animals were treated with the vehicle. At the end of the treatment period, blood, liver and adipose tissue samples were collected from the euthanized animals. The results show that PG extract significantly decreased (P < 0.05) HSL activity in adipose tissue and liver of diabetic animals which was accompanied by increased glycogen levels, reduced serum triglycerides, total cholesterol, LDL-cholesterol and increased HDL-cholesterol. This study demonstrates that P. guajava has significant anti-diabetic effects that include increased glycogen storage and reduced HSL activity in the liver and adipose tissue with an improved serum lipid profile.
糖尿病的特征是由于胰岛素分泌或胰岛素作用缺陷导致的高血糖，并伴有一般代谢紊乱。番石榴叶具有降血糖作用，已被证实具有降血糖作用。本研究旨在探讨番石榴叶提取物对糖原合酶（GS）和糖原磷酸化酶（GP）组织活性、激素敏感脂肪酶（HSL）组织活性、血清脂质谱以及组织损伤血清酶生物标志物的影响。雄性 Sprague-Dawley 大鼠一次性给予 40mg/kg 体重链脲佐菌素诱导糖尿病。用番石榴叶的水提取物治疗正常和糖尿病动物（400mg/kg 体重）2 周，而对照动物用载体治疗。在治疗期末，从安乐死动物采集血液、肝脏和脂肪组织样本。结果表明，PG 提取物显著降低（P<0.05）糖尿病动物脂肪组织和肝脏中的 HSL 活性，同时糖原水平升高，血清三酰甘油、总胆固醇、LDL-胆固醇降低，HDL-胆固醇升高。这项研究表明，番石榴具有显著的抗糖尿病作用，包括增加肝和脂肪组织中的糖原储存和降低 HSL 活性，以及改善血清脂质谱。
