甘油和三乙胺两步培养杜氏盐藻积累油脂：缓解三乙胺对生物量抑制作用的可行方法。
Two-Stage Cultivation of Dunaliella tertiolecta with Glycerol and Triethylamine for Lipid Accumulation: a Viable Way To Alleviate the Inhibitory Effect of Triethylamine on Biomass.
机构信息
College of Food Science and Engineering, South China University of Technology, Guangzhou, China.
Industrial Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, China.
出版信息
Appl Environ Microbiol. 2019 Feb 6;85(4). doi: 10.1128/AEM.02614-18. Print 2019 Feb 15.
Microalgae are promising alternatives for sustainable biodiesel production. Previously, it was found that 100 ppm triethylamine greatly enhanced lipid production and lipid content per cell of by 20% and 80%, respectively. However, triethylamine notably reduced biomass production and pigment contents. In this study, a two-stage cultivation with glycerol and triethylamine was attempted to improve cell biomass and lipid accumulation. At the first stage with 1.0 g/liter glycerol addition, cells reached the late log phase in a shorter time due to rapid cell growth, leading to the highest cell biomass (1.296 g/liter) for 16 days. However, the increased glycerol concentrations with glycerol addition decreased the lipid content. At the second-stage cultivation with 100 ppm triethylamine, the highest lipid concentration and lipid weight content were 383.60 mg/liter and 37.7% of dry cell weight (DCW), respectively, in the presence of 1.0 g/liter glycerol, which were 27.36% and 72.51% higher than those of the control group, respectively. Besides, the addition of glycerol alleviated the inhibitory effect of triethylamine on cell morphology, algal growth, and pigment accumulation in The results indicated that two-stage cultivation is a viable way to improve lipid yield in microalgae. Microalgae are promising alternatives for sustainable biodiesel production. Two-stage cultivation with glycerol and triethylamine enhanced the lipid productivity of , indicating that two-stage cultivation is an efficient strategy for biodiesel production from microalgae. It was found that glycerol significantly enhanced cell biomass of , and the presence of glycerol alleviated the inhibitory effect of triethylamine on algal growth. Glycerol, the major byproduct from biodiesel production, was used for the biomass accumulation of at the first stage of cultivation. Triethylamine, as a lipid inducer, was used for lipid accumulation at the second stage of cultivation. Two-stage cultivation with glycerol and triethylamine enhanced lipid productivity and alleviated the inhibitory effect of triethylamine on the algal growth of , which is an efficient strategy for lipid production from .
微藻是可持续生物柴油生产的有前途的替代品。此前发现，100ppm 的三乙胺可分别将 的产脂量和单位细胞内脂含量提高 20%和 80%。然而，三乙胺显著降低了生物质产量和色素含量。在这项研究中，尝试了使用甘油和三乙胺进行两阶段培养，以提高细胞生物质和脂质积累。在第一阶段添加 1.0g/L 甘油时，由于细胞快速生长，细胞在较短时间内进入对数后期，从而在 16 天内达到最高细胞生物量（1.296g/L）。然而，随着甘油浓度的增加，添加甘油会降低脂质含量。在第二阶段培养中添加 100ppm 的三乙胺时，在 1.0g/L 甘油存在下，最高脂质浓度和脂质重量含量分别为 383.60mg/L 和干细胞重量（DCW）的 37.7%，分别比对照组高 27.36%和 72.51%。此外，甘油的添加缓解了三乙胺对细胞形态、藻类生长和色素积累的抑制作用。结果表明，两阶段培养是提高微藻产脂率的可行方法。微藻是可持续生物柴油生产的有前途的替代品。使用甘油和三乙胺进行两阶段培养可提高 的脂质生产力，表明两阶段培养是从微藻生产生物柴油的有效策略。结果发现，甘油显著提高了 的细胞生物质，并且甘油的存在缓解了三乙胺对藻类生长的抑制作用。甘油是生物柴油生产的主要副产物，在培养的第一阶段用于 细胞生物质的积累。三乙胺作为脂质诱导剂，在培养的第二阶段用于脂质积累。使用甘油和三乙胺进行两阶段培养可提高脂质生产力，并缓解三乙胺对 的藻类生长的抑制作用，这是从 生产脂质的有效策略。
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