College of Food Science and Engineering, South China University of Technology , Guangzhou 510640, China.
Institute of Hydrobiology, Chinese Academy of Sciences , Wuhan 430072, China.
J Agric Food Chem. 2017 Apr 19;65(15):3190-3197. doi: 10.1021/acs.jafc.7b00552. Epub 2017 Apr 10.
Dunaliella tertiolecta, a halotolerant alga, can accumulate large amounts of neutral lipid, which makes it a potential biodiesel feedstock. In this study, neutral lipids of D. tertiolecta induced by different salinities or N or P starvation were analyzed by thin-layer chromatography (TLC), flow cytometry (FCM), and confocal laser scanning microscopy (CLSM). High salinities or N or P starvation resulted in a decrease in cell growth and chlorophyll contents of D. tertiolecta. Neutral lipid contents increased markedly after 3-7 days of N starvation or at low NaCl concentrations (0.5-2.0 M). N starvation had a more dramatic effect on the neutral lipid contents of D. tertiolecta than P starvation. Four putative ME isozymes in different conditions can be detected by using isozyme electrophoresis. Two alternative acetyl-CoA producers, ACL and ACS genes, were up-regulated under low salinities and N starvation. It was suggested that low salinities and N starvation are considered efficient ways to stimulate lipid accumulation in D. tertiolecta.
杜氏盐藻是一种耐盐藻类，可以积累大量中性脂质，因此是一种有潜力的生物柴油原料。本研究采用薄层层析（TLC）、流式细胞术（FCM）和共聚焦激光扫描显微镜（CLSM）分析了不同盐度或氮、磷饥饿诱导的杜氏盐藻中性脂质。高盐度或氮、磷饥饿导致杜氏盐藻细胞生长和叶绿素含量下降。氮饥饿或在低 NaCl 浓度（0.5-2.0 M）下培养 3-7 天后，中性脂质含量显著增加。氮饥饿对杜氏盐藻中性脂质含量的影响比磷饥饿更为显著。不同条件下可以通过同工酶电泳检测到 4 种假定的 ME 同工酶。在低盐度和氮饥饿下，两个替代乙酰辅酶 A 产生酶，ACL 和 ACS 基因被上调。这表明，低盐度和氮饥饿被认为是刺激杜氏盐藻脂质积累的有效方法。
