Akgönüllü Semra, Özgür Erdoğan, Denizli Adil
Department of Chemistry, Division of Biochemistry, Hacettepe University, Ankara 06800, Turkey.
Micromachines (Basel). 2022 Sep 1;13(9):1441. doi: 10.3390/mi13091441.
Aptamers are important materials for the specific determination of different disease-related biomarkers. Several methods have been enhanced to transform selected target molecule-specific aptamer bindings into measurable signals. A number of specific aptamer-based biosensors have been designed for potential applications in clinical diagnostics. Various methods in combination with a wide variety of nano-scale materials have been employed to develop aptamer-based biosensors to further increase sensitivity and detection limit for related target molecules. In this critical review, we highlight the advantages of aptamers as biorecognition elements in biosensors for target biomolecules. In recent years, it has been demonstrated that electrode material plays an important role in obtaining quick, label-free, simple, stable, and sensitive detection in biological analysis using piezoelectric devices. For this reason, we review the recent progress in growth of aptamer-based QCM biosensors for medical diagnoses, including virus, bacteria, cell, protein, and disease biomarker detection.
适体是用于特异性测定不同疾病相关生物标志物的重要材料。已经改进了几种方法，以将选定的靶分子特异性适体结合转化为可测量的信号。已经设计了许多基于特异性适体的生物传感器，用于临床诊断的潜在应用。已经采用了各种与多种纳米级材料相结合的方法来开发基于适体的生物传感器，以进一步提高对相关靶分子的灵敏度和检测限。在这篇综述中，我们强调了适体作为生物传感器中用于靶生物分子的生物识别元件的优势。近年来，已经证明电极材料在使用压电器件的生物分析中获得快速、无标记、简单、稳定和灵敏的检测方面起着重要作用。因此，我们综述了基于适体的QCM生物传感器用于医学诊断（包括病毒、细菌、细胞、蛋白质和疾病生物标志物检测）的最新进展。
