Department of Endocrinology, Xiangya Hospital of Central South University, Changsha, 410005, China.
Department of Endocrinology, Xiangya Hospital of Central South University, Changsha, 410005, China.
Biochem Biophys Res Commun. 2019 Jan 22;508(4):1145-1148. doi: 10.1016/j.bbrc.2018.12.051. Epub 2018 Dec 13.
Numerous studies have provided that long noncoding RNAs (lncRNAs) possess important roles in regulating tumorigenesis. However, up to data, the role of LINC00514 in cancer, including thyroid cancer, remains unknown. In the present study, we found that LINC00514 expression was significantly upregulated in papillary thyroid cancer (PTC) tissues by bioinformatics analysis. Loss-of-function studies revealed that LINC00514 silencing inhibited the proliferation, migration and invasion of PTC cells while promoting apoptosis in vitro. Moreover, LINC00514 knockdown suppressed PTC growth in vivo. RNA-FISH showed that LINC00514 mainly locates in the nucleus of PTC cells. Through bioinformatics prediction, we identified that LINC00514 served as the sponge for miR-204-3p, and miR-204-3p directly targeted CDC23. Thus, LINC00514 promoted CDC23 expression via restraining miR-204-3p activity, leading to PTC progression. In sum, our findings demonstrated that LINC00514 contributes to PTC progression and might be a potential target for PTC therapy.
大量研究表明，长链非编码 RNA（lncRNAs）在调控肿瘤发生中具有重要作用。然而，截至目前，LINC00514 在癌症中的作用，包括甲状腺癌，仍然未知。在本研究中，我们通过生物信息学分析发现 LINC00514 在甲状腺乳头状癌（PTC）组织中表达显著上调。功能丧失研究表明，LINC00514 沉默抑制 PTC 细胞的增殖、迁移和侵袭，同时促进细胞凋亡。此外，LINC00514 敲低抑制体内 PTC 的生长。RNA-FISH 显示 LINC00514 主要位于 PTC 细胞的核内。通过生物信息学预测，我们鉴定出 LINC00514 作为 miR-204-3p 的海绵，而 miR-204-3p 直接靶向 CDC23。因此，LINC00514 通过抑制 miR-204-3p 的活性促进 CDC23 的表达，导致 PTC 的进展。总之，我们的研究结果表明，LINC00514 促进 PTC 的进展，可能是 PTC 治疗的潜在靶点。
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