AKT/mTOR 信号通路是通过 LINC00514/miR-28-5p/TRIM44 轴介导的。
The AKT/mTOR Signaling Pathway Was Mediated through the LINC00514/miR-28-5p/TRIM44 Axis.
机构信息
Department of the Central Laboratory, The Second Affiliated Hospital of Xi'an Medical University, Xi'an 710038, China.
Department of Pharmacology, Shaanxi University of Chinese Medicine, Xianyang 712046, China.
出版信息
Dis Markers. 2022 Sep 23;2022:1889467. doi: 10.1155/2022/1889467. eCollection 2022.
OBJECTIVE
Increasing evidence has demonstrated the essential role of lncRNAs in tumorigenesis. LINC00514, a novel lncRNA, was reported to be a promoter of malignant behaviors in cancer, but in pituitary adenoma (PA), its biological functions remain unclear.
METHODS
Herein, we measured LINC00514 expression by RT-qPCR analysis which indicated a significant elevation of LINC00514 expression in human PA tissues. Moreover, the effect of LINC00514 silencing on PA cell proliferation and invasion was, respectively, examined by CCK-8 and transwell assays.
RESULTS
The results showed that LINC00514 deletion markedly inhibited PA cell proliferation and invasion. Besides, investigation on the molecular mechanisms showed that LINC00514 might function as an endogenous RNA (ceRNA) to sponge miR-28-5p and TRIM44 was mediated by LINC00514-derived miR-28-5p in PA cells. Furthermore, the AKT/mTOR signaling pathway was mediated through the LINC00514/miR-28-5p/TRIM44 axis.
CONCLUSION
To sum up, we suggested LINC00514 as a novel therapeutic target for PA treatment.
目的
越来越多的证据表明 lncRNAs 在肿瘤发生中起着重要作用。LINC00514 是一种新的 lncRNA，据报道它在癌症中促进恶性行为，但在垂体腺瘤（PA）中，其生物学功能尚不清楚。
方法
在此，我们通过 RT-qPCR 分析来测量 LINC00514 的表达，结果表明 LINC00514 在人 PA 组织中的表达显著升高。此外，通过 CCK-8 和 Transwell 测定分别检测了 LINC00514 沉默对 PA 细胞增殖和侵袭的影响。
结果
结果表明，LINC00514 的缺失显著抑制了 PA 细胞的增殖和侵袭。此外，对分子机制的研究表明，LINC00514 可能作为内源性 RNA（ceRNA）通过 LINC00514 衍生的 miR-28-5p 来海绵 miR-28-5p，并在 PA 细胞中介导 TRIM44。此外，AKT/mTOR 信号通路是通过 LINC00514/miR-28-5p/TRIM44 轴介导的。
结论
综上所述，我们认为 LINC00514 可以作为 PA 治疗的新的治疗靶点。
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