College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou 310058, People's Republic of China; Institute of Bioengineering, Zhejiang Academy of Medical Sciences, Hangzhou 310013, People's Republic of China.
College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou 310058, People's Republic of China.
Virology. 2019 Feb;528:37-47. doi: 10.1016/j.virol.2018.12.004. Epub 2018 Dec 13.
In vertebrates, pyroptosis is an intense inflammatory form of programmed cell death. This death pathway is critical for controlling pathogenic infection. In invertebrates, however, due to the lack of adaptive immune response, it is still elusive whether Caspase 1-dependent cell death pathway exists. In this study, our data showed that Caspase 1-mediated cell death was activated by white spot syndrome virus to counteract virus infection. Caspase 1 had a higher expression in hemocytes and lymphoid-like organ in shrimp and WSSV infection was promoted upon the inhibition of Caspase 1 enzymatic activity. IL-1β-like protein was identified as the substrate of Caspase 1 and its interaction with Caspase 1 was validated ectopically and endogenously. Moreover, IL-1β like protein was released into extracellular contents under WSSV infection and Prophenoloxidase system was activated, resulting in the reduction of WSSV. Our data unraveled a previously unidentified mechanism through which Caspase 1-dependent cell death controlled virus infection in shrimp.
在脊椎动物中，细胞焦亡是一种强烈的炎症性程序性细胞死亡形式。该死亡途径对于控制病原感染至关重要。然而，在无脊椎动物中，由于缺乏适应性免疫反应，仍然难以确定是否存在 Caspase 1 依赖性细胞死亡途径。在这项研究中，我们的数据表明，Caspase 1 介导的细胞死亡被白斑综合征病毒激活，以对抗病毒感染。在虾中，Caspase 1 在血细胞和淋巴样器官中的表达较高，并且 Caspase 1 的酶活性受到抑制后，WSSV 感染会得到促进。IL-1β 样蛋白被鉴定为 Caspase 1 的底物，其与 Caspase 1 的相互作用在外源和内源水平均得到了验证。此外，在 WSSV 感染下，IL-1β 样蛋白被释放到细胞外内容物中，同时激活了酚氧化酶系统，导致 WSSV 数量减少。我们的数据揭示了一个以前未被识别的机制，即 Caspase 1 依赖性细胞死亡控制虾中的病毒感染。
