Instituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de investigaciones Científicas y Tecnológicas (CONICET), Los Reseros y Nicolas Repetto (1686), Hurlingham, Buenos Aires, Argentina.
Instituto de Microbiología y Zoología Agrícola, Centro de Investigación en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), Los Reseros y Nicolas Repetto (1686), Hurlingham, Buenos Aires, Argentina.
J Appl Microbiol. 2019 Mar;126(3):811-825. doi: 10.1111/jam.14176. Epub 2019 Jan 21.
Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value-added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost-efficient enzymatic cocktails.
We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo- and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β-xylosidase, a β-glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10.
Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes.
Cellulomonas sp. B6 could serve as a source of lignocellulose-degrading enzymes applicable to bioprocessing and biotechnological industries.
木质纤维素生物质的解构是获得生物燃料和增值产品的瓶颈。我们的主要目标是表征新型分离株 Cellulomonas sp. B6 的分泌组，当在剩余生物质上生长以制定具有成本效益的酶鸡尾酒时。
我们在 Cellulomonas sp. B6 的基因组中鉴定了 205 种潜在的 CAZymes，其中 91 种是糖苷水解酶（GH）。通过对在膨化麦秸（EWS）、磨碎甘蔗秸秆（SCR）或羧甲基纤维素（CMC）中培养的上清液的分泌组分析，我们确定了在木质纤维素解构中起作用的蛋白质。在 CMC 上生长导致分泌两种外切葡聚糖酶（GH6 和 GH48）和两种 GH10 木聚糖酶，而在 SCR 或 EWS 上生长导致鉴定出多种 CAZymes。从预测分泌的 32 种 GH 中，有 22 种在 EWS 和/或 SCR 培养物的上清液中被鉴定出来，包括内切葡聚糖酶和外切葡聚糖酶、木聚糖酶、木葡聚糖酶、阿拉伯呋喃糖苷酶/β-木糖苷酶、β-葡萄糖苷酶和 AA10。令人惊讶的是，在木聚糖酶中，有七种是 GH10。
Cellulomonas sp. B6 在木质纤维素生物质上的生长诱导了多种 CAZymes 的分泌。
Cellulomonas sp. B6 可以作为适用于生物加工和生物技术行业的木质纤维素降解酶的来源。
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