Noireau F, Lemesre J L, Nzoukoudi M Y, Louembet M T, Gouteux J P, Frezil J L
Laboratoire d'Entomologie Médicale et de Parasitologie, Centre ORSTOM de Brazzaville, Republic of the Congo.
Trans R Soc Trop Med Hyg. 1988;82(2):237-40. doi: 10.1016/0035-9203(88)90430-0.
The card agglutination test for trypanosomiasis (CATT) was evaluated and compared to the classical immunofluorescent antibody test (IFAT) in the immunological diagnosis of Gambian trypanosomiasis. Tests were performed on serum and whole blood. Cross-reactions were found in the CATT with sera from patients suffering from parasitic infections other than sleeping sickness, but could be largely overcome by selecting 1/10 as the specific threshold dilution. At 1/40 dilution no false positive result was observed in the IFAT. At the specific threshold dilution, the sensitivity of IFAT was 94.7%, compared with 91.6% for the CATT. On whole blood, a more convenient sample in the field, IFAT specificity (100%) was greater than that of the CATT (94.3%), as was its sensitivity (92% compared with 82.5%). In view of its simplicity and rapidity of execution, the CATT is an efficient serological test to detect new foci. When greater sensitivity is required, IFAT should be preferred to CATT.
在冈比亚锥虫病的免疫诊断中，对锥虫病卡片凝集试验（CATT）进行了评估，并与经典的免疫荧光抗体试验（IFAT）进行了比较。对血清和全血进行了检测。在CATT中发现，患有昏睡病以外寄生虫感染患者的血清会出现交叉反应，但通过选择1/10作为特异性阈值稀释度，这种交叉反应在很大程度上可以克服。在1/40稀释度时，IFAT未观察到假阳性结果。在特异性阈值稀释度下，IFAT的灵敏度为94.7%，而CATT为91.6%。对于全血（一种在现场更方便采集的样本），IFAT的特异性（100%）高于CATT（94.3%），其灵敏度也是如此（分别为92%和82.5%）。鉴于其操作简单快速，CATT是检测新疫点的一种有效血清学检测方法。当需要更高的灵敏度时，应优先选择IFAT而非CATT。
